Binding Affinity of Sulfonic Acid Dyes with Plasma Proteins
| Dye | No. of -SO3H groups | No. of atoms between -SO3H groups | % bound to plasma proteins* |
|---|---|---|---|
| Methylene blue | 0 | 0 | 0.0 ± 0.0 |
| Phenol red | 1 | 0 | 10.0 ± 1.2 |
| Patent blue VF | 2 | 1 | 4.7 ± 1.2 |
| Sulforhodamine 101 | 2 | 1 | 14.2 ± 1.0 |
| Orange G | 2 | 1 | 15.7 ± 2.1 |
| Acid yellow 42 | 2 | 2 | 84.2 ± 3.1 |
| Naphthol blue black | 2 | 3 | 99.7 ± 1.9 |
| Nitrazine yellow | 2 | 3 | 100.6 ± 1.3 |
| Chrysophenine | 2 | 4 | 96.2 ± 2.4 |
| Direct yellow 27 | 2 | 5 | 97.9 ± 5.8 |
| Reactive blue 4 | 2 | 6 | 102.8 ± 7.9 |
| Indigo carmine | 2 | 8 | 28.0 ± 2.3 |
| Potassium indigotetrasulfonate | 4 | 1 and 6 | 12.7 ± 0.8 |
| Evans blue | 4 | 1 and 20 | 68.1 ± 3.5 |
| 99mTc-Evans blue | 4 | 1 and 20 | 69.6 ± 2.5 |
| Chicago sky blue | 4 | 1 and 20 | 69.3 ± 1.5 |
| 99mTc-Chicago sky blue | 4 | 1 and 20 | 70.8 ± 0.6 |
| Trypan blue | 4 | 3 and 14 | 62.2 ± 2.0 |
| 99mTc-Trypan blue | 4 | 3 and 14 | 60.5 ± 0.7 |
| Direct yellow | 4 | 4 and 19 | 58.6 ± 2.4 |
↵* Mean ± SD.