Effect of Various Anesthetic Procedures on Eppendorf Microelectrode Measurements Obtained from Individual Dunning R3327-AT Tumors*
| Animal no. (gender) | Tumor weight (g) | Restrained | Anesthetized with halothane and | Ketamine-xylazine | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| O2 | Carbogen | Air | |||||||||
| A | B | A | B | A | B | A | B | A | B | ||
| 1 (F) | 10.7 | 35.4 | 19.8 | 0 | 24.7 | 0 | 18.3 | 0 | 3.2 | 59.4 | |
| 2 (F) | 3.0 | 59.4 | 14.2 | 0 | 24.5 | 0 | 18.8 | 0 | 4.0 | 50.8 | |
| 3 (F) | 6–10† | 2.0 | 82.3 | 18.3 | 0.8 | 13.7 | 0 | 15.3 | 0 | 1.1 | 89.1 |
| 4 (F) | 4.3 | 51 | 12.8 | 0 | 14.4 | 0 | 13.1 | 0 | |||
| 5 (F) | 7.0 | 37.2 | 21.9 | 0 | 19.1 | 0 | 22 | 0 | |||
| 6 (F) | 2.4 | 70.2 | 25.2 | 0 | 28.8 | 0 | 26.3 | 0 | 4.7 | 56.0 | |
| 7 (F) | 4.2 | 7.6 | 42.1 | 9.0 | 16.7 | 5.0 | 49.0 | 10.5 | 15.9 | ||
| 8 (F) | 5.7 | 20.9 | 0 | 20.3 | 0 | 22.4 | 0 | 9.9 | 13.7 | 6.7 | 29.4 |
| 9 (F) | 7.7 | 3.5 | 85.0 | 13.3 | 12.9 | 5.4 | 41.2 | 4.1 | 70.6 | 6.8 | 14.7 |
| 10 (F) | 7.1 | 2.0 | 85.0 | 11.8 | 2.0 | 6.6 | 10.8 | 9.2 | 22.5 | 6.2 | 5.9 |
| 11 (F) | 4.8 | 3.7 | 86.6 | 13.1 | 0 | 10.4 | 4.0 | 12.9 | 0 | 4.5 | 54.9 |
| 12 (M) | 4.4 | 13.7 | 2.7 | 17.5 | 0 | 8.2 | 0 | 37.8 | 0 | 7.6 | 3.6 |
| 13 (M) | 6.6 | 13.6 | 0 | 7.6 | 21.6 | 12.2 | 17.6 | 10.7 | 12.5 | 6.2 | 23.5 |
| 14 (F) | 3.1 | 7.2 | 32.2 | 32.2 | 4.2 | 4.3 | 58.8 | 19.3 | 8.5 | 6.1 | 18.8 |
| 15 (M) | 5.6 | 10.9 | 0 | 21.0 | 3.5 | 19.1 | 0 | 33.7 | 0 | 4.6 | 60.8 |
| Mean | 6.5 | 7.5 | 44.6 | 17.2 | 4.1 | 14.6 | 12.1 | 16.7 | 9.6 | 5.1 | 38.9 |
| ±SE | 0.4 | 1.4 | 8.6 | 1.7 | 1.8 | 2.1 | 5.3 | 2.6 | 4.8 | 0.5 | 7.7 |
| Increase from restrained value | 13/15 | 11/15 | 12/15 | 5/12 | |||||||
| Decrease from restrained value | 2/15 | 4/15 | 3/15 | 7/12 | |||||||
| Mean‡ | 4.3 | 63.1 | 17.2 | 3.7 | 13.2 | 3.2 | 15.2 | 11.8 | 4.8 | 41.5 | |
| ±SE | 0.7 | 6.8 | 2.3 | 1.9 | 2.7 | 1.1 | 2.2 | 7.0 | 0.7 | 11.2 | |
| Increase from restrained value | 10/10 | 8/10 | 10/10 | 5/7 | |||||||
| Decrease from restrained value | 0/10 | 2/10 | 0/10 | 2/7 | |||||||
* A, median Po2 in mm Hg (reading varied from 99 to 125); B, percentage of measurements <5 mm Hg.
↵† These tumor weights were estimated from caliper measurements. Tumors in animals 7 through 15 were excised and weighed at end of each experiment.
↵‡ Means from those tumors whose median Po2 values under conditions of restraint were <10 mm Hg.