Average Number of DOTA-Biotin Groups per Carrier Molecule and Conditions of Immunization
| Carrier | Groups per molecule (n) | Biotin/protein (molar ratio) | Dose of injection (mol × 10−9/kg) | Type of injection |
|---|---|---|---|---|
| TOC | 1.0 | * | 42 (L)/420 (H) | i.p. |
| ChiMov19 | 6.0 | 2.66 | 2.7 (L)/27 (H) | i.p./i.p. or s.c. |
| Mov19 | 5.0 | 4.12 | 2.7 (L)/27 (H) | i.p./i.p. or s.c. |
| † | * | 27 (H) | i.p. | |
| MGR6 | † | * | 27 (H) | i.p. |
| Human serum albumin | 3.0 | 3.10 | ‡ | ‡ |
↵* Not biotinylated, because not used as target for enzyme-linked immunosorbent assay (ELISA) or radioimmunoassay (RIA).
↵† Not derivatized with DOTA; used to verify immunogenicity of carrier.
↵‡ Not injected; used as target for ELISA and RIA.
L = low dose; H = high dose; i.p. = intraperitoneal; s.c. = subcutaneous.
Data for TOC are as reported by Paganelli et al. (4). TOC was used also in clinical study, from 50 to 84 mol × 10−9/kg.