Oligonucleotides Used for PCR Amplifications
| Gene | Quantification method | Sequence | cDNA |
|---|---|---|---|
| MDR1 | Forward primer | 5′CCCAGGAGCCCATCCTGT3′ | 3774-3791 |
| Reverse primer | 5′CCCGGCTGTTGTCTCCATA3′ | 3838-3821 | |
| Probe | 5′(FAM)TGACTGCAGCATTGCTGAGAACATTGC(TAMRA)3′ | 3793-3819 | |
| MRP | Forward primer | 5′AAGCGCCTCGAGTCGGT3′ | 3617-3633 |
| Reverse primer | 5′TCGAATGACGCTGACCCC3′ | 3694-3677 | |
| Probe | 5′(FAM)AGCCGCTCCCCGGTCTATTCCC(TAMRA)3′ | 3635-3656 | |
| LRP | Forward primer | 5′TTTCGATGACTTCCATAAGAACTCA3′ | 1881-1905 |
| Reverse primer | 5′TTCCGAGGTCTCAAAGCCAA3′ | 1950-1931 | |
| Probe | 5′(FAM)CCCGCATCATTCGCACTGCTGT(TAMRA)3′ | 1907-1928 | |
| GAPDH | Forward primer | 5′GAAGGTGAAGGTCGGAGTCA3′ | |
| Reverse primer | 5′GAAGATGGTGATGGGA3′ | ||
| Probe | 5′(JOE)CAAGCTTCCCGTTCTCAGCC(TAMRA)3′ |