HPLC Analysis of Effluent Samples from Isolated Rat Liver with FOP
| Group | Intact FOP (%) | Eluted metabolites (%) | Retained metabolites (%) |
|---|---|---|---|
| Normoxic (n = 3) | |||
| Early (0.5–2 min) | 69.0 ± 10.3 | 30.5 ± 9.7 | 0.5 ± 2.2 |
| Late (11–13 min) | 66.0 ± 14.1 | 20.2 ± 13.3 | 13.8 ± 1.2* |
| Hypoxic (n = 3) | |||
| Early (0.5–2 min) | 91.4 ± 1.0† | 9.1 ± 1.7† | −0.5 ± 2.1 |
| Late (11–13 min) | 89.7 ± 5.6† | 8.8 ± 4.8† | 1.0 ± 2.3† |
↵* P < 0.0001 vs. early phase.
↵† P < 0.01 vs. normoxic.
FOP was administered to livers as bolus. Effluent was sampled during washout of radioactivity from livers over early and late intervals given in table. HPLC conditions were as given in Materials and Methods. Fractions of radioactivity as intact FOP, eluted metabolites, and metabolites retained on C-18 HPLC column (obtained by balance of first 2) are given.