Analysis of Fc–Fc Receptor Binding by Surface Plasmon Resonance (Biacore T200)
| Antibody | KD (M) murine FcγRIV | Relative murine FcγRIV binding | KD (M) human FcγRIIIa-158V | Relative human FcγRIIIa-158V binding |
|---|---|---|---|---|
| HuE71-IgG1 | 7.24E−07 | 1.00 | 8.73E−07 | 1.00 |
| Desferrioxamine-HuE71-IgG1 | 5.39E−07 | 1.34 | 6.64E−07 | 1.31 |
| HuE71-IgG1-Afuco | 2.27E−07 | 3.19 | 2.86E−07 | 3.05 |
| Desferrioxamine-HuE71- IgG1-Afuco | 2.19E−07 | 3.31 | 2.94E−07 | 2.96 |
| HuE71-IgG1-Aglyco | NB | — | NB | — |
| Desferrioxamine-HuE71- IgG1-Aglyco | NB | — | NB | — |
KD (M) = equilibrium dissociation constant; NB = no binding.