Extractions Performed on Brain Tissue for ELISA Analysis
| Step | Material | Extraction | Medium | Centrifugation |
|---|---|---|---|---|
| 1 | Fresh-frozen brain tissue | 1:5 weight:volume ratio of tissue | TBS | 1 h, 16,000g |
| 2 | Pellet TBS extraction (step 1) | 1:5 weight:volume ratio of tissue | 70% formic acid | 1 h, 16,000g |
| 3 | TBS extract (step 1) | 200 μL of TBS extract (step 1) | TBS | 1 h, 100,000g |