PT  - JOURNAL ARTICLE
AU  - Gyu Seong Heo
AU  - Geetika Bajpai
AU  - Wenjun Li
AU  - Hannah P. Luehmann
AU  - Deborah H. Sultan
AU  - Hao Dun
AU  - Florian Leuschner
AU  - Steven L. Brody
AU  - Robert J. Gropler
AU  - Daniel Kreisel
AU  - Kory J. Lavine
AU  - Yongjian Liu
TI  - Targeted PET Imaging of Chemokine Receptor 2–Positive Monocytes and Macrophages in the Injured Heart
AID  - 10.2967/jnumed.120.244673
DP  - 2021 Jan 01
TA  - Journal of Nuclear Medicine
PG  - 111--114
VI  - 62
IP  - 1
4099  - http://jnm.snmjournals.org/content/62/1/111.short
4100  - http://jnm.snmjournals.org/content/62/1/111.full
SO  - J Nucl Med2021 Jan 01; 62
AB  - Proinflammatory macrophages are important mediators of inflammation after myocardial infarction and of allograft injury after heart transplantation. The aim of this study was to image the recruitment of proinflammatory chemokine receptor 2–positive (CCR2+) cells in multiple heart injury models. Methods: 64Cu-DOTA-extracellular loop 1 inverso (ECL1i) PET was used to image CCR2+ monocytes and macrophages in a heart transplantation mouse model. Flow cytometry was performed to characterize CCR2+ cells. Autoradiography on a human heart specimen was conducted to confirm binding specificity. 64Cu- and 68Ga-DOTA-ECL1i were compared in an ischemia–reperfusion injury mouse model. Results: 64Cu-DOTA-ECL1i showed sensitive and specific detection of CCR2+ cells in all tested mouse models, with efficacy comparable to that of 68Ga-DOTA-ECL1i. Flow cytometry demonstrated specific expression of CCR2 on monocytes and macrophages. The tracer binds to human CCR2. Conclusion: This work establishes the utility of 64Cu-DOTA-ECL1i to image CCR2+ monocytes and macrophages in mouse models and provides the requisite preclinical information to translate the targeted clinical-grade CCR2 imaging probe for clinical investigation of heart diseases.