TY - JOUR T1 - FAPI-74 PET/CT Using Either <sup>18</sup>F-AlF or Cold-kit <sup>68</sup>Ga-labeling: Biodistribution, Radiation Dosimetry and Tumor Delineation in Lung Cancer Patients JF - Journal of Nuclear Medicine JO - J Nucl Med DO - 10.2967/jnumed.120.245084 SP - jnumed.120.245084 AU - Frederik Giesel AU - Sebastian Adeberg AU - Mustafa Syed AU - Thomas Lindner AU - Luis David Jimenez AU - Eleni Mavriopoulou AU - Fabian Staudinger AU - Eric Tonndorf-Martini AU - Sebastian Regnery AU - Stefan Rieken AU - Rami ElShafie AU - Manuel Röhrich AU - Paul Flechsig AU - Andreas Kluge AU - Annette Altmann AU - Juergen Debus AU - Uwe A. Haberkorn AU - Clemens Kratochwil Y1 - 2020/09/01 UR - http://jnm.snmjournals.org/content/early/2020/09/18/jnumed.120.245084.abstract N2 - 68Ga-FAPI-2/4/46 have already been proposed as promising PET-tracers. However, the short half-life of 68Ga (T1/2 68 min) creates problems with manufacture and delivery. 18F (T1/2 110 min) labeling would result in a more practical large scale production and a cold-kit formulation would improve the spontaneous availability. The NOTA-chelator ligand FAPI-74 can be labeled with both 18F-AlF (Aluminum-Fluoride) and 68Ga. Here we describe the in-vivo evaluation of 18F-FAPI-74 and a proof-of-mechanism of 68Ga-FAPI-74 labeled at ambient temperature. Methods: In ten patients with lung cancer PET-scans were acquired at 10 min, 1h and 3H after administration of 259±26 MBq 18F-FAPI-74. Physiological biodistribution and tumor uptake were semi-quantitatively evaluated based on SUV at each time-point. Absorbed doses were evaluated using OLINDA/EXM 1.1 and QDOSE dosimetry software with the dose calculator IDAC-Dose 2.1. Identical methods were used to evaluate one exam after injection of 263 MBq 68Ga-FAPI-74. Results: The highest contrast was achieved 1 h p.i. in primary tumors, lymph node and distant metastases with SUVmax &gt;10, respectively. The effective dose per 100 MBq administered activity of 18F-FAPI-74 was 1.4±0.2 mSv and for 68Ga-FAPI-74 it was 1.6 mSv. Thus, the radiation burden of a diagnostic 18F-FAPI-74 PET-scan is even lower than that of PET-scans with 18F-FDG and other 18F-tracers; 68Ga-FAPI-74 is comparable to other 68Ga-ligands. FAPI-PET/CT supported target volume definition for guiding radiotherapy. Conclusion: High contrast and low radiation burden of FAPI-74 PET/CT favors multiple clinical applications. Centralized large-scale production of 18F-FAPI-74 or decentralized cold-kit labeling of 68Ga-FAPI-74 allows flexible routine use. ER -