RT Journal Article
SR Electronic
T1 Intracellular Distribution and Radiotoxicity of Chromium-51 in Mammalian Cells: Auger-Electron Dosimetry
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 59
OP 67
VO 26
IS 1
A1 A. I. Kassis
A1 K. S. R. Sastry
A1 S. J. Adelstein
YR 1985
UL http://jnm.snmjournals.org/content/26/1/59.abstract
AB The kinetics of uptake and of radiotoxicity of chromium-51, an Auger-electron emitter, have been studied in V79 lung fibroblasts of the Chinese hamster. Intracellular radioactivity was directly proportional to the incubation period and to the extracellular concentration f the Cr-51. About 14% of the cellular activity was associated with the nucleus, whereas approximately 2% was guanidine-precipitable and therefore bound to DNA. The growth rate of V79 cells was slowed following intracellular incorporation of Cr-51. The cell-survival curve, in terms of colony-forming ability, was of the low-LET type, with a D37 of 6.2 pCi/cell. Theoretical dosimetric estimates indicate that, under the given experimental conditions, the mean lethal dose to the cell nucleus was 870 rad. Although this value is somewhat larger than the x-ray D37 dose of 580 rad for this cell line, it is more realistic than the gross underestimate obtained by classical MIRD calculations (2–3 rad/cell).