PT  - JOURNAL ARTICLE
AU  - A. I. Kassis
AU  - K. S. R. Sastry
AU  - S. J. Adelstein
TI  - Intracellular Distribution and Radiotoxicity of Chromium-51 in Mammalian Cells: Auger-Electron Dosimetry
DP  - 1985 Jan 01
TA  - Journal of Nuclear Medicine
PG  - 59--67
VI  - 26
IP  - 1
4099  - http://jnm.snmjournals.org/content/26/1/59.short
4100  - http://jnm.snmjournals.org/content/26/1/59.full
SO  - J Nucl Med1985 Jan 01; 26
AB  - The kinetics of uptake and of radiotoxicity of chromium-51, an Auger-electron emitter, have been studied in V79 lung fibroblasts of the Chinese hamster. Intracellular radioactivity was directly proportional to the incubation period and to the extracellular concentration f the Cr-51. About 14% of the cellular activity was associated with the nucleus, whereas approximately 2% was guanidine-precipitable and therefore bound to DNA. The growth rate of V79 cells was slowed following intracellular incorporation of Cr-51. The cell-survival curve, in terms of colony-forming ability, was of the low-LET type, with a D37 of 6.2 pCi/cell. Theoretical dosimetric estimates indicate that, under the given experimental conditions, the mean lethal dose to the cell nucleus was 870 rad. Although this value is somewhat larger than the x-ray D37 dose of 580 rad for this cell line, it is more realistic than the gross underestimate obtained by classical MIRD calculations (2–3 rad/cell).