RT Journal Article
SR Electronic
T1 Development of a Sensitive Radioassay of Histamine for In vitro Allergy Testing
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 56
OP 63
VO 25
IS 1
A1 Faraj, Bahjat A.
A1 Gottlieb, George R.
A1 Camp, Vernon M.
A1 Kutner, Michael
A1 Lolies, Pat
YR 1984
UL http://jnm.snmjournals.org/content/25/1/56.abstract
AB A radioenzymatic assay for the measurement of histamine is described, based on the incubation of histamine in the presence of histamine-N-methyl-transferase from rat kidney and [3H-methyl]-S-adenosyl-L-methionine (sp act 15 Ci/mmol) in phosphate buffer, 0.05 mole/I, pH 7.9, at 37°C for 60 min. The N-[3H]-methyl]histamine generated was selectively extracted into toluene/isoamyl alcohol (3:2) and the quantity of the tritium in the sample was determined by liquid-scintillation counting. As little as 1 nmol/I of histamine can be detected. The assay is specific, with no cross-reactivity noted for several compounds closely related to histamine. The assay was used to measure the released histamine of a group of allergic subjects following the incubation of their blood with various allergens. A good correlation was found between histamine release from whole blood and the response of skin mast cells to intradermal antigen administration.