@article {Faraj56, author = {Bahjat A. Faraj and George R. Gottlieb and Vernon M. Camp and Michael Kutner and Pat Lolies}, title = {Development of a Sensitive Radioassay of Histamine for In vitro Allergy Testing}, volume = {25}, number = {1}, pages = {56--63}, year = {1984}, publisher = {Society of Nuclear Medicine}, abstract = {A radioenzymatic assay for the measurement of histamine is described, based on the incubation of histamine in the presence of histamine-N-methyl-transferase from rat kidney and [3H-methyl]-S-adenosyl-L-methionine (sp act 15 Ci/mmol) in phosphate buffer, 0.05 mole/I, pH 7.9, at 37{\textdegree}C for 60 min. The N-[3H]-methyl]histamine generated was selectively extracted into toluene/isoamyl alcohol (3:2) and the quantity of the tritium in the sample was determined by liquid-scintillation counting. As little as 1 nmol/I of histamine can be detected. The assay is specific, with no cross-reactivity noted for several compounds closely related to histamine. The assay was used to measure the released histamine of a group of allergic subjects following the incubation of their blood with various allergens. A good correlation was found between histamine release from whole blood and the response of skin mast cells to intradermal antigen administration.}, issn = {0161-5505}, URL = {https://jnm.snmjournals.org/content/25/1/56}, eprint = {https://jnm.snmjournals.org/content/25/1/56.full.pdf}, journal = {Journal of Nuclear Medicine} }