TY - JOUR T1 - <strong>Effects of remote ischemic conditioning on myocardial perfusion, myocardial viability, left ventricular function and remodeling in pigs with acute myocardial infarction evaluated by serially gated <sup>99m</sup>Tc-MIBI SPECT/CT and <sup>18</sup>F-FDG PET/CT</strong> JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 220 LP - 220 VL - 61 IS - supplement 1 AU - Yao Lu AU - Jing Tian AU - Yi Tian AU - Yihan Zhou AU - Wanwan Wen AU - Ziwei Zhu AU - Xiang Li AU - Xiaoli Zhang Y1 - 2020/05/01 UR - http://jnm.snmjournals.org/content/61/supplement_1/220.abstract N2 - 220Objectives: We aim to address regional cardiac alterations assessed by radionuclide imaging with radiopharmaceuticals targeting different molecular processes to predict the dynamics of left ventricular (LV) remodeling following acute myocardial infarction (AMI) in Chinese mini-pigs. Methods: AMI was induced by placing an embolus into 1 to 2 mm under the first anterior descending branch in 10 animals. In the experimental group, remote ischemic conditioning was induced in pigs by blood pressure inflation on the lower limbs for 5-min period and 4 cycles after AMI. A series of gated 99mTc-MIBI myocardial perfusion SPECT/CT (G-SPECT) imaging and gated 18F-FDG (3.89 ± 0.4MBq) PET/CT imaging (G-PET) were performed longitudinally at 3rd, 14th and 28th day after AMI. Total perfusion defect (TPD, %), hibernating myocardium (HM, %), scar myocardium (%), LV global function (LVEF, %), LV remodeling (EDV, ESV) and regional function (wall motion and wall thickening) were calculated and analyzed. One-way ANOVA was used to compare the significance of differences of various parameters at different time points (P &lt;0.05), all parametric data were expressed as mean ± SD. Results: One pig absenting AMI and one pig died 14th day after surgery were excluded. A total of 8 pigs (34.0±1.7kg) were finally assessed. As shown in Table 1, in the experimental group (n=5), both TPD (44.2 ± 7.0%, 20.6 ± 8.9%, 19.8 ± 5.0%, P&lt;0. 01) and the extent of HM (23.4 ± 9.0%, 8.6 ± 9.0%, 8.0 ± 5.6%, P&lt;0.05) as well as scar (20.8 ± 8.5%, 11.8 ± 4.5%, 11.6 ± 4.4%, P&lt;0.05) was significantly decreased with time prolonged after the surgery; and LVEF was increased on 14th day and sustained at 28th day (38.2 ± 18.5%, 60.4 ± 6.7%, 55.8 ± 17.2%, P &lt;0.05). No significant adverse myocardial remodeling (EDV, ESV) was observed on 14th day and 28th day (P&gt;0.05). Both SMS and STS decreased on 14th day whereas subsequently increased on 28th day (P&gt;0.05). In the control group (n=3), both ESV (22.7 ± 7.5mL, 32.3 ± 8.5mL, 36.0 ± 10.1mL, P &lt; 0.05) and EDV (42.0 ± 3.5mL, 57.7 ± 11.9mL, 63.0 ± 10.8mL, P &lt; 0.05) were gradually increased after surgery. Correspondingly, LVEF (58.3 ± 9.0%, 49.7 ± 8.3%, 43.7 ± 6.7%, P &lt;0.05) was gradually reduced after surgery. TPD, HM, SMS and STS were increased on 14th day and sustained at 28th day, scar was gradually increased without statistical significance (P&gt;0.05). Conclusions: Remote ischemic conditioning was a simple technique which has a specific protective effect on the AMI model. It could improve LV function in the subacute phase and prevent the development of LV remodeling. Acknowledgements: This project was sponsored by the National Natural Science Foundation of (81871377, 81571717), Capital Characteristic Clinical Application Research (Z181100001718071). View this table:Serial left ventricular function and remodeling at different time after surgery in each group ER -