RT Journal Article
SR Electronic
T1 In vivo PET imaging of neuroinflammation response after intravenous transplantation of olfactory ensheathing cells in a hemisection spinal cord injury rat model
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 213
OP 213
VO 61
IS supplement 1
A1 Lijian Zhang
A1 Xiaoqing Zhuang
A1 Thomas Keller
A1 Anna Krzyczmonik
A1 Paivi Kotitalo
A1 Sarita Forsback
A1 Tove Gronroos
A1 Chunlei Han
A1 Merja Haaparanta-Solin
A1 Olof Solin
A1 Francisco López Picón
A1 Hechun Xia
YR 2020
UL http://jnm.snmjournals.org/content/61/supplement_1/213.abstract
AB 213Introduction: In vivo PET imaging of neuroinflammation response after intravenous transplantation of olfactory ensheathing cells in a hemisection spinal cord injury rat model. Objectives: Neuroinflammation is a prominent neuropathological feature of spinal cord injury (SCI). Our previous study showed that intravenous transplantation of olfactory ensheathing cells (OECs) can decrease neuroinflammation after SCI. In this study, in vivo PET/CT imaging and ex vivo spinal cord autoradiography using the TSPO targeting radiotracer [18F]F-DPA (Keller et al., http://dx.doi.org/10.1177/0271678X19853117) was used to image neuroinflammation in a hemisection SCI rat model with and without intravenous transplantation of OECs. Methods: Male Sprague-Dawley rats underwent a lateral hemisection on the twelfth thoracic (T12) vertebra and were randomly divided to OEC group (n = 3/timepoint) and vehicle group (n = 3/timepoint). At 1 day after the surgery, OECs were intravenously transplanted to the OEC rats, while the rats in the vehicle group received culture medium. [18F]F-DPA PET/CT imaging was performed 1, 7, and 14 days after transplantation. The uptake of [18F]F-DPA was quantitatively assessed as standardized uptake value ratio (SUVr), between the mean SUV at the injury region (T11-T13) and the mean SUV at a reference region (C2-C4). After PET imaging, the spinal cord around the injury site was collected for autoradiography analysis. The immunohistochemical studies were performed on the same spinal cord slices to quantify the expression levels of Iba-1 and TSPO. Results: In the OEC group, the SUVr values of [18F]F-DPA decreased with time. In the vehicle group, the SUVr values peaked at 7 days after transplantation. The autoradiography analyses showed that the uptake of [18F]F-DPA in the vehicle group was significantly higher than in the OEC group at 7 days after transplantation (p = 0.025). The expression of Iba-1 and TSPO in the vehicle group was also significantly higher than in the OEC group (p = 0.008; p < 0.0001 respectively). Conclusions: The intravenous transplantation of OECs can mediate activated microglia to inhibit neuroinflammation after SCI. Acknowledgements: This work was supported by the Natural Science Fund of the Ningxia Hui Autonomous Region (2018AAC02012, 2018BCG01002).