RT Journal Article
SR Electronic
T1 TSPO Versus P2X7 as a Target for Neuroinflammation: An In Vitro and In Vivo Study
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 604
OP 607
DO 10.2967/jnumed.119.231985
VO 61
IS 4
A1 Van Weehaeghe, Donatienne
A1 Van Schoor, Evelien
A1 De Vocht, Joke
A1 Koole, Michel
A1 Attili, Bala
A1 Celen, Sofie
A1 Declercq, Lieven
A1 Thal, Dietmar R.
A1 Van Damme, Philip
A1 Bormans, Guy
A1 Van Laere, Koen
YR 2020
UL http://jnm.snmjournals.org/content/61/4/604.abstract
AB Neuroinflammation is important in amyotrophic lateral sclerosis (ALS). The P2X7 receptor (P2X7R) is a promising target for neuroinflammation. The objective of this study was to compare 18F-DPA714, a second-generation translocator protein tracer, with 11C-JNJ717, a novel P2X7R tracer, in vitro and in vivo in ALS. Methods: For the in vitro portion of the study, autoradiography with 18F-DPA714 and 11C-JNJ717 was performed on human ALS brain sections in comparison to immunofluorescence with Iba1 and GFAP. For the in vivo portion, 3 male patients with early-stage ALS (59.3 ± 7.2 y old) and 6 healthy volunteers (48.2 ± 16.5 y old, 2 men and 4 women) underwent dynamic PET/MR scanning with 18F-DPA714 and 11C-JNJ717. Volume-of-distribution images were calculated using Logan plots and analyzed on a volume-of-interest basis. Results: Autoradiography showed no difference in 11C-JNJ717 binding but did show increased 18F-DPA714 binding in the motor cortex correlating with Iba1 expression (glial cells). Similar findings were observed in vivo, with a 13% increase in 18F-DPA714 binding in the motor cortex. Conclusion: In symptomatic ALS patients, 18F-DPA714 showed increased signal whereas 11C-JNJ717 was not elevated.