RT Journal Article
SR Electronic
T1 Small-Animal <sup>18</sup>F-FDG PET for Research on <em>Octopus vulgaris</em>: Applications and Future Directions in Invertebrate Neuroscience and Tissue Regeneration
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1302
OP 1307
DO 10.2967/jnumed.117.205393
VO 59
IS 8
A1 Letizia Zullo
A1 Ambra Buschiazzo
A1 Michela Massollo
A1 Mattia Riondato
A1 Alessia Democrito
A1 Cecilia Marini
A1 Fabio Benfenati
A1 Gianmario Sambuceti
YR 2018
UL http://jnm.snmjournals.org/content/59/8/1302.abstract
AB This study aimed to develop a method of administering 18F-FDG to the common octopus in order to perform a PET biodistribution assay characterizing glucose metabolism in organs and regenerating tissues. Methods: Seven animals (two of which had a regenerating arm) were anesthetized with 3.7% MgCl2 in artificial seawater and then injected with 18–30 MBq of isosmotic 18F-FDG through either the left branchial heart or the anterior vena cava. After an uptake time of about 50 min, the animals were sacrificed and placed on the bed of a small-animal PET scanner, and 10-min static acquisitions were obtained at 3–4 bed positions to visualize the entire body. To confirm image interpretation, internal organs of interest were collected and counted with a γ-counter. Results: Administration through the anterior vena cava resulted in a good full-body distribution of 18F-FDG as seen on the PET images. Uptake was high in the mantle mass and relatively lower in the arms. In particular, the brain, optic lobes, and arms were clearly identified and were measured for their uptake (SUVmax: 6.57 ± 1.86, 7.59 ± 1.66, and 1.12 ± 0.06, respectively). Interestingly, 18F-FDG uptake was up to 3-fold higher in the highly proliferating areas of regenerating arms. Conclusion: This study represents a stepping-stone to the use of noninvasive functional techniques for addressing questions about invertebrate neuroscience and regenerative medicine.