PT - JOURNAL ARTICLE AU - Yafu Yin AU - Qi Wang AU - Yao Diao TI - <strong>Preliminary study of the relationship between nicotine improving cognitive function and nicotine-induced anti-inflammatory effect in ischemia rats</strong> DP - 2018 May 01 TA - Journal of Nuclear Medicine PG - 271--271 VI - 59 IP - supplement 1 4099 - http://jnm.snmjournals.org/content/59/supplement_1/271.short 4100 - http://jnm.snmjournals.org/content/59/supplement_1/271.full SO - J Nucl Med2018 May 01; 59 AB - 271Objectives: To investigate the correlation between nicotine improving cognitive function and anti-inflammatory mechanism of nicotine through the JAK2-STAT3 signaling pathway. Methods: The focal ischemic cognitive impairment model was established by injection of endothelin -1 (ET-1) into the left thalamus area of rats. After intraperitoneal injection of Nicotine (1.5mg/kg/d), AG490 (3 mg/kg/d), nicotine (1.5mg/kg/d) +AG490 (3 mg/kg/d) and saline for 9 consecutive days, they were divided into nicotine group, AG490 group, nicotine+AG490 and the ischemic group. The Morris water maze (MWM) test was used to detect the cognitive function of rats in the fourth day after operation. At the end of the study, 2-[18F]-A-85380 Micro PET imaging was performed immediately. The effects of nicotine on alpha 4 beta 2 nAChRs receptor subunits, inflammatory factors and JAK2-STAT3 signaling pathway were detected by Western blot technique. Results: In MWM navigation test, the spatial learning and memory ability of nicotine group was significantly higher than ischemic group, nicotine+AG490 group and AG490 group (all p&lt;0.05). There was no significant difference between nicotine +AG490 group and AG490 group compared with ischemic group (p&gt;0.05). In MWM experiment in space exploration, the memory ability of nicotine group rats compared with ischemia group rats was obviously improved too (p&lt;0.05). There was no significant difference between nicotine+AG490 group and AG490 group compared with ischemic group (p&gt;0.05). Micro PET imaging was performed after injection of 2-[18F]-A-85380 120 min. The uptake of 2-[18F]-A-85380 in different brains domains in nicotine group and nicotine+AG490 group were all higher than those in ischemic group and AG490 groups. Western blot results showed that the expression level of alpha 4 nAChR, beta 2 nAChR, p-jak2 and p-stat3 in the left thalamus in nicotine group was significantly higher than that in ischemia group. In nicotine+AG490 and AG490 group, the expression of p-stat3 and p-jak2 protein was decreased than nicotine group, there was no significant difference compared with ischemia group; in nicotine+AG490 group, the expression of alpha 4 nAChR and beta 2 nAChR protein was significantly higher than that in the ischemia group, while there was no significant difference compared with the nicotine group. The expression of IL-1 beta and IL-6 protein in the left thalamus of nicotine group was lower than that in the other three groups (all p&lt;0.05). There was no significant difference in the relative expression of inflammatory factors between the AG490 group and the nicotinic+AG490 group compared with the ischemic group. Conclusion: Nicotine intervention improving cognitive function in ischemia rats might be associated with nicotine-induced anti-inflammatory effect by activating JAK2-STAT3 signaling pathway.