RT Journal Article
SR Electronic
T1 Construction of radioactive iodine nanoparticle for active targeting and therapy of anaplastic thyroid cancer
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1290
OP 1290
VO 59
IS supplement 1
A1 Linlin Zhang
A1 Hui Wang
YR 2018
UL http://jnm.snmjournals.org/content/59/supplement_1/1290.abstract
AB 1290Objectives: To observe whether the radioactive iodine labeled thyroglobin which modified by RGD nanoparticles (131I-nTG-RGD) has the therapy effect to ATC. Methods: ATC cell ARO incubated into the armpit of nude mice to make tumorigenicity. we explored nTG and achieved remarkably prolonged circulation of protein in vivo, whose outer layer was decorated by RGD. In vitro immunocyte test was used to detect whether nanocapsules can evade the attacks of internal immune system. 125I radiolabeled targeting nanocapsules injected into the anaplastic thyroid carcinoma mice via tail vein. We also used SPECT to image the biodistribution of nano-capsules and calculate biological half-life; radioactive nanocapsules of NS group, simple 131I group, 131I-nTG group, 131I-nTG-RGD group were injected into the ATC mice through the tail vein, respectively. Tumor size of each groups were measured every day, meanwhile tumor curves and %ID/g of each organs were recorded everyday. The therapeutic of nanoparticle on ATC were also assessed. Results: nTG was synthesized successfully, and tested through AGE, DLS, zeta potential measurement approachs. In vitro test confirmed that RGD-nTG nanoparticle targeting synthesized by RGD which based on MPC can evade the immunocyte phagocytosis. Compared to normal saline group, simple 131I group, 131I-nTG group, tumors in 131I-nTG-RGD nanocapsules radioactive targeting group grow slower , and smaller increasement in volume, with significant statistical differences in vivo experiments (P<0.01). Conclution: 131I-nTG-RGD was made successfully based on MPC. This targeted radioactive nanocapsules can achieve long circulation in vivo and significantly inhibit the migration and proliferation of the anaplastic thyroid cancer.