RT Journal Article
SR Electronic
T1 The firstApplication of 68Ga labeled ssDNAAptamer Sgc8 in Colorectal Patients
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 53
OP 53
VO 59
IS supplement 1
A1 Mingru Zhang
A1 Zhe Wang
A1 Shuailiang Wang
A1 Weidong Yang
A1 Jing Wang
A1 Xiaoyuan Chen
YR 2018
UL http://jnm.snmjournals.org/content/59/supplement_1/53.abstract
AB 53Purpose: The protein tyrosine kinase-7 (PTK7) is overexpressed in various types of human cancers.As a specific imaging agent of PTK7, 68Ga-Sgc8 was investigated in this study to assess its application in colorectal patients. Methods: Three patients underwent 68Ga-Sgc8 PET/CT scans. For each patient, 1.11-1.48 MBq (0.03-0.04 mCi) per kilogram of 68Ga-Sgc8 was injected intravenously. A low-dose CT scan (CARE Dose4D, 4[asterisk]1.2 mm layer, 512 × 512 matrix, 70 cm FOV) was performed 40 min later, followed by whole-body PET acquisition with six beds from vertex to toes, 2 min in duration. PET/CT images were independently reviewed by 3 experienced nuclear medicine physicians. Resected tissues from all three patients were sliced, followed by immunohistochemical staining to evaluate the expression of PTK7. Results: Four lesions were resected from the three colorectal patients & two of which were conformed to be malignant with the maximum standardized uptake values (SUVmax) of 3.70 and 3.07, which were higher than those of benign tissues (1.50 and 1.00), suggesting that 68Ga-Sgc8 PET/CT could differentially diagnose malignancy from benign tumors and normal tissues. The histopathological staining confirmed that PTK7 was overexpressed on malignancy and barely expressed in benign tumors. Conclusions: With a favorable distribution profile, 68Ga-Sgc8 is safe and tolerable for clinical imaging. 68Ga-Sgc8 PET/CT can not only identify colorectal cancer, but also have a potential for the differential diagnosis of benign and malignant, suggesting that 68Ga-Sgc8 may be a promising tracer in the application of colorectal cancer diagnosis.Research Grant: This work was supported by the Key Program of National Natural Science Foundation of China (Grant No. 81230033), the Major State Basic Research Development Program (Grant No. 2011CB707704), the Major Instrument of National Natural Science Foundation Research Project (Grant No. 81227901), the National Natural Science Foundation of China (Grant No. 81371594, 81401442, 81501501, 81671713, 81601521), the Foundation Program of Xijing Hospital (Grant No. XJZT15G01, XJZT15M07, XJZT15ZC05), Shaanxi Science & Technology Co-ordination & Innovation Project, China (Grant No. 2016KTCQ03-09).