RT Journal Article
SR Electronic
T1 Monitor the Progression of Chronic Liver Damage In Rats Using 18F-PBR06
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1287
OP 1287
VO 59
IS supplement 1
A1 Shuo Huang
A1 Hui Wang
YR 2018
UL http://jnm.snmjournals.org/content/59/supplement_1/1287.abstract
AB 1287Objectives: Accurate and rapid assessment liver condition is the key of therapy for hepatitis patients.This study aim to evaluate PBR radioligand [18F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline (18F-PBR06) as a positron emission tomography (PET) imaging biomarker of chronic liver damage in a rat model. Methods: Rat liver damage model was constructed by bile duct ligation (BDL) which initiates a complex cascade of pathological events that leads to cholestasis and inflammation resulting in a strong fibrotic and severe hepatocyte injury. PET scanning, immunohistochemical staining, HE staining,masson staining and qRT-PCR were performed to elucidate the relationships among quantify PBR levels,radioactivity uptake and liver injury in chronic inflammatory rat model. Results: AST, ALT and TBIL increased after BDL and peaked in 1 week,then gradually dropped along with time till 4 weeks, which was still higher than control(All P<0.05). PET and histological results showed that the uptake of radioactivity in livers increased continuously in 1, 2, 3, and 4 weeks ()with disease progression.Further, the mRNA levels of the functional macromolecular signaling complex composed of PBR were obviously higher compared to controls. Conclusions: PBR is highly expressed in injury hepatocytes and macrophages in BDL liver.18F-PBR06 can serve as an sensitive probe to damage in liver. 18F-PBR06 imaging may be used to accurately assess conditions in liver and guide the therapeutic schedule,especially for those patients with severe liver disease but have normal or mildly elevated serum ALT and AST.As a non-invasive testing measure, 18F-PBR06 PET scans could be a choice for patients who is unwilling to accepte liver biopsy.<!--EndFragment-->