@article {Xiao2113, author = {Wenchang Xiao and Huanhuan Yan and Lu Wan}, title = {Effects of fasting duration on mouse intestinal imaging of 18F-FDG}, volume = {59}, number = {supplement 1}, pages = {2113--2113}, year = {2018}, publisher = {Society of Nuclear Medicine}, abstract = {2113Purpose: 18F-Fludeoxyglucose (18F-FDG) is a powerful radiotracer for Positron Emission Tomography (PET) scanning that has been wildly used in numerous researches and diagnostic applications, such as oncology, neurology, cardiology, and so on. Although 18F-FDG has been proved to be an ideal indicator of glucose metabolic status, the most appropriate procedures for intestinal imaging of 18F-FDG still reminds unclear. Since 18F-FDG uptake is highly affected by the fasting duration, we have focused on how the fasting duration affects the intestinal imaging of 18F-FDG. Materials and Methods: Mouse model: 6 adult male wild type C57BL/6J mice were equally divided into two groups: group A and group B. Mice of each group were fed in one cage together for 3 months. Reagents: 2-Deoxy-2-(18F)fluoro-D-glucose (18F-FDG) Equipments: Trans-PET {\textregistered} BioCaliburn {\textregistered} 700 (Raycan Technology Co., Ltd, Suzhou, China) Methods: Group A and group B mice were fasted before 18F-FDG administration and PET scanning. In the first week, group A mice were fasted for 15 hours (hr) from 18:00 pm to 9:00 am in the next day, and group B mice were fasted for 6 hours from 8:00 am to 14:00 pm. Then Mice were injected with 18F-FDG via tail vein 60 minutes (min) before they were placed on the PET bed for 14min PET static scanning (7min/bed, 2 beds, inhaled anesthesia with 2\% isoflurane). In the second week, group B mice were fasted for 15hr from 18:00 pm to 9:00 am in the next day, and group A mice were fasted for 6hr from 8:00 am to 14:00 pm. Then the following operations were administered as above. In the following 4 weeks, mice of group A and group B received 15hr and 6hr fasting as above in turns until both group were processed 3 times for both fasting duration. Last, images were analyzed together. Results: 18F-FDG PET scanning images of each group in the same experiment showed that intestinal images of the same group were similar. As mice fed in same cage were usually with the parallel gut microbiota, the similarity above was reasonable. We also found that imaging quality and SUV(Standard Uptake Value) of 3 times experiences of each group with same fasting duration were stable. Interestingly, 18F-FDG PET images of fasting duration for 15hr were more distinct t than that of 6hr of the same group. Conclusion: Intestinal 18F-FDG PET scanning images are similar in mice fed in the same cage, and stable for multiple testing during a short period, such as in one month. More importantly, 15hr fasting duration overnight is better than 6hr for intestinal 18F-FDG PET imaging.}, issn = {0161-5505}, URL = {https://jnm.snmjournals.org/content/59/supplement_1/2113}, eprint = {https://jnm.snmjournals.org/content}, journal = {Journal of Nuclear Medicine} }