RT Journal Article SR Electronic T1 In Vitro Evaluation of Molecular Tumor Targets in Nuclear Medicine: Immunohistochemistry Is One Option, but Under Which Conditions? JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 1885 OP 1887 DO 10.2967/jnumed.117.197582 VO 58 IS 12 A1 Jean Claude Reubi YR 2017 UL http://jnm.snmjournals.org/content/58/12/1885.abstract AB The identification of new molecular targets for diagnostic and therapeutic applications using in vitro methods is an important challenge in nuclear medicine. One such method is immunohistochemistry, increasingly popular because it is easy to perform. This review presents the case for conducting receptor immunohistochemistry to evaluate potential molecular targets in human tumor tissue sections. The focus is on the immunohistochemistry of G-protein–coupled receptors, one of the largest families of cell surface proteins, representing a major class of drug targets and thus playing an important role in nuclear medicine. This review identifies common pitfalls and challenges and provides guidelines on performing such immunohistochemical studies. An appropriate validation of the target is a prerequisite for developing robust and informative new molecular probes.