PT  - JOURNAL ARTICLE
AU  - Reubi, Jean Claude
TI  - In Vitro Evaluation of Molecular Tumor Targets in Nuclear Medicine: Immunohistochemistry Is One Option, but Under Which Conditions?
AID  - 10.2967/jnumed.117.197582
DP  - 2017 Dec 01
TA  - Journal of Nuclear Medicine
PG  - 1885--1887
VI  - 58
IP  - 12
4099  - http://jnm.snmjournals.org/content/58/12/1885.short
4100  - http://jnm.snmjournals.org/content/58/12/1885.full
SO  - J Nucl Med2017 Dec 01; 58
AB  - The identification of new molecular targets for diagnostic and therapeutic applications using in vitro methods is an important challenge in nuclear medicine. One such method is immunohistochemistry, increasingly popular because it is easy to perform. This review presents the case for conducting receptor immunohistochemistry to evaluate potential molecular targets in human tumor tissue sections. The focus is on the immunohistochemistry of G-protein–coupled receptors, one of the largest families of cell surface proteins, representing a major class of drug targets and thus playing an important role in nuclear medicine. This review identifies common pitfalls and challenges and provides guidelines on performing such immunohistochemical studies. An appropriate validation of the target is a prerequisite for developing robust and informative new molecular probes.