RT Journal Article
SR Electronic
T1 Performing Repeated Quantitative Small-Animal PET with an Arterial Input Function Is Routinely Feasible in Rats
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 611
OP 616
DO 10.2967/jnumed.116.182402
VO 58
IS 4
A1 Huang, Chi-Cheng
A1 Wu, Chun-Hu
A1 Huang, Ya-Yao
A1 Tzen, Kai-Yuan
A1 Chen, Szu-Fu
A1 Tsai, Miao-Ling
A1 Wu, Hsiao-Ming
YR 2017
UL http://jnm.snmjournals.org/content/58/4/611.abstract
AB Performing quantitative small-animal PET with an arterial input function has been considered technically challenging. Here, we introduce a catheterization procedure that keeps a rat physiologically stable for 1.5 mo. We demonstrated the feasibility of quantitative small-animal 18F-FDG PET in rats by performing it repeatedly to monitor the time course of variations in the cerebral metabolic rate of glucose (CMRglc). Methods: Aseptic surgery was performed on 2 rats. Each rat underwent catheterization of the right femoral artery and left femoral vein. The catheters were sealed with microinjection ports and then implanted subcutaneously. Over the next 3 wk, each rat underwent 18F-FDG quantitative small-animal PET 6 times. The CMRglc of each brain region was calculated using a 3-compartment model and an operational equation that included a k*4. Results: On 6 mornings, we completed 12 18F-FDG quantitative small-animal PET studies on 2 rats. The rats grew steadily before and after the 6 quantitative small-animal PET studies. The CMRglc of the conscious brain (e.g., right parietal region, 99.6 ± 10.2 μmol/100 g/min; n = 6) was comparable to that for 14C-deoxyglucose autoradiographic methods. Conclusion: Maintaining good blood patency in catheterized rats is not difficult. Longitudinal quantitative small-animal PET imaging with an arterial input function can be performed routinely.