TY - JOUR T1 - Intratumorally Injected <sup>177</sup>Lu-Labeled Gold Nanoparticles: Gold Nanoseed Brachytherapy with Application for Neoadjuvant Treatment of Locally Advanced Breast Cancer JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 936 LP - 942 DO - 10.2967/jnumed.115.168906 VL - 57 IS - 6 AU - Simmyung Yook AU - Zhongli Cai AU - Yijie Lu AU - Mitchell A. Winnik AU - Jean-Philippe Pignol AU - Raymond M. Reilly Y1 - 2016/06/01 UR - http://jnm.snmjournals.org/content/57/6/936.abstract N2 - Improvements in the treatment of locally advanced breast cancer (LABC) are needed. Our objective was to study a radiation nanomedicine (gold nanoseeds) composed of 30-nm gold nanoparticles (AuNP) modified with polyethyleneglycol (PEG) chains linked to DOTA for complexing the β-particle emitter 177Lu and to panitumumab for targeting epidermal growth factor receptors (EGFR) (177Lu-T-AuNP) as a novel neoadjuvant brachytherapy for LABC. Nontargeted gold nanoseeds (177Lu-NT-AuNP) were constructed without panitumumab for comparison. Methods: 177Lu-T-AuNP or 177Lu-NT-AuNP was injected intratumorally in CD-1 athymic mice bearing subcutaneous EGFR-positive MDA-MB-468 human breast cancer tumors. Biodistribution and small-animal SPECT/CT imaging studies were performed to evaluate tumor and normal organ localization. A short-term (15 d) study was conducted to select the most effective amount of 177Lu-T-AuNP or 177Lu-NT-AuNP for treatment with long-term observation (90–120 d). Normal organ toxicities were assessed by monitoring body weight, blood cell counts, and serum alanine aminotransferase and creatinine. Radiation-absorbed doses in the tumor and normal organs were estimated by Monte Carlo N-Particle version 5.0 modeling. Results: Tumor radioactivity concentrations were high at 1 h after injection (&gt;300–400 percentage injected dose per gram [%ID/g]) but decreased by 2–3-fold at 48 h after injection. Normal organ uptake was low (&lt;0.5 %ID/g) except for the liver and spleen (&lt;3 %ID/g), increasing by 2–5-fold at 48 h after injection. Treatment with 4.5 MBq (6 × 1011 AuNP) of 177Lu-T-AuNP or 177Lu-NT-AuNP arrested tumor growth over 90 d without normal organ toxicity, whereas tumors continued to grow in mice treated with unlabeled T-AuNP or 177Lu-labeled PEG polymer not linked to AuNP. Survival was prolonged up to 120 d in mice treated with 177Lu-T-AuNP or 177Lu-NT-AuNP. Radiation-absorbed doses to the tumor were 30 and 22 Gy for 177Lu-T-AuNP and 177Lu-NT-AuNP, respectively. Some tumor regions received high radiation doses (250–1,300 Gy). Normal organ doses were low (0.04–0.6 Gy). Conclusion: Gold nanoseeds injected intratumorally were highly effective for inhibiting the growth of breast cancer tumors in CD-1 athymic mice and caused no normal organ toxicity. These results are promising for their application for neoadjuvant brachytherapy of LABC. Because EGFR targeting was not required, the approach is broadly applicable to LABC with different phenotypes. ER -