RT Journal Article SR Electronic T1 Multimodality imaging of dual-labeled human mitochondria for cardioprotection in the ischemic heart JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 451 OP 451 VO 57 IS supplement 2 A1 Vamsidhar Akurathi A1 Douglas Cowan A1 James Inkster A1 Rouan Yao A1 Erin Snay A1 Jerusha Thedsanamoorthy A1 Yaotang Wu A1 Pedro Nido A1 S. Ted Treves A1 James McCully A1 Alan Packard YR 2016 UL http://jnm.snmjournals.org/content/57/supplement_2/451.abstract AB 451Objectives Transplantation of mitochondria offers a unique therapeutic approach for cardioprotection in the patients suffering from myocardial infarction. In previous studies we showed that transplantation of autologously derived, respiration-competent, mitochondria significantly improved contractile function of the post-ischemic heart (Am J Physiol Heart Circ Physiol.2013, 304, H966-982). As part of our on-going investigation of the underlying biochemistry of this process, here we report the dynamics of dual-labeled (PET/MRI) human cardiac fibroblast derived mitochondria in ischemic and perfused isolated rabbit hearts.Methods The purified mitochondria (1x108) in the respiration media were labeled with 18F-rhodamine 6G (18F-R6G, ~ 0.2MBq/mL) and 30 nm iron oxide particles, and injected through the coronary vasculature of isolated rabbit hearts. After reperfusion and fixation, the distribution of the transplanted mitochondria in the global or regional ischemic hearts was determined by CT/PET/MRI. The localization of transplanted mitochondria in the hearts was confirmed by histology with a mitochondria-specific stain.Results The retention of the dual-labeled mitochondria in the ischemic and perfused hearts was found to be > 98%. The CT/PET/MR imaging showed the labeled mitochondria distribution of the regional and ischemic hearts. The histology showed that the transplanted mitochondria were located in the interstitial spaces and to some extent internalized by the cardiomyocytes. .Conclusions These results demonstrate that coronary artery perfusion of dual-labeled mitochondria results in a heterogenous distribution of mitochondria throughtout the heart. Additional experiments are ongoing to study the real-time kinetics of the labeled mitochondria. Research Support: NIH-5R01HL108107, Children’s Hospital Radiology Foundation, Richard A. and Susan F. Smith, Presidents Innovation Award, Boston Children’s Hospital, Michael B. Klein and Family