PT - JOURNAL ARTICLE AU - Vamsidhar Akurathi AU - Douglas Cowan AU - James Inkster AU - Rouan Yao AU - Erin Snay AU - Jerusha Thedsanamoorthy AU - Yaotang Wu AU - Pedro Nido AU - S. Ted Treves AU - James McCully AU - Alan Packard TI - Multimodality imaging of dual-labeled human mitochondria for cardioprotection in the ischemic heart DP - 2016 May 01 TA - Journal of Nuclear Medicine PG - 451--451 VI - 57 IP - supplement 2 4099 - http://jnm.snmjournals.org/content/57/supplement_2/451.short 4100 - http://jnm.snmjournals.org/content/57/supplement_2/451.full SO - J Nucl Med2016 May 01; 57 AB - 451Objectives Transplantation of mitochondria offers a unique therapeutic approach for cardioprotection in the patients suffering from myocardial infarction. In previous studies we showed that transplantation of autologously derived, respiration-competent, mitochondria significantly improved contractile function of the post-ischemic heart (Am J Physiol Heart Circ Physiol.2013, 304, H966-982). As part of our on-going investigation of the underlying biochemistry of this process, here we report the dynamics of dual-labeled (PET/MRI) human cardiac fibroblast derived mitochondria in ischemic and perfused isolated rabbit hearts.Methods The purified mitochondria (1x108) in the respiration media were labeled with 18F-rhodamine 6G (18F-R6G, ~ 0.2MBq/mL) and 30 nm iron oxide particles, and injected through the coronary vasculature of isolated rabbit hearts. After reperfusion and fixation, the distribution of the transplanted mitochondria in the global or regional ischemic hearts was determined by CT/PET/MRI. The localization of transplanted mitochondria in the hearts was confirmed by histology with a mitochondria-specific stain.Results The retention of the dual-labeled mitochondria in the ischemic and perfused hearts was found to be > 98%. The CT/PET/MR imaging showed the labeled mitochondria distribution of the regional and ischemic hearts. The histology showed that the transplanted mitochondria were located in the interstitial spaces and to some extent internalized by the cardiomyocytes. .Conclusions These results demonstrate that coronary artery perfusion of dual-labeled mitochondria results in a heterogenous distribution of mitochondria throughtout the heart. Additional experiments are ongoing to study the real-time kinetics of the labeled mitochondria. Research Support: NIH-5R01HL108107, Children’s Hospital Radiology Foundation, Richard A. and Susan F. Smith, Presidents Innovation Award, Boston Children’s Hospital, Michael B. Klein and Family