RT Journal Article SR Electronic T1 Immuno-PET Imaging of CD30-Positive Lymphoma Using 89Zr-Desferrioxamine–Labeled CD30-Specific AC-10 Antibody JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 96 OP 102 DO 10.2967/jnumed.115.162735 VO 57 IS 1 A1 Svetlana N. Rylova A1 Luigi Del Pozzo A1 Cathrin Klingeberg A1 Roswitha Tönnesmann A1 Anna L. Illert A1 Philipp T. Meyer A1 Helmut R. Maecke A1 Jason P. Holland YR 2016 UL http://jnm.snmjournals.org/content/57/1/96.abstract AB The CD30-specific antibody–drug conjugate, brentuximab vedotin, is approved for the treatment of relapsed, refractory Hodgkin lymphomas and systemic anaplastic large T-cell lymphomas. Multiple ongoing clinical trials are investigating brentuximab vedotin efficacy in other CD30-positive hematologic malignancies. Because CD30 expression varies among different types of lymphoma and can also change during the course of treatment, companion diagnostic imaging of CD30 could be a valuable tool in optimizing patient-specific brentuximab vedotin treatment regimens. Methods: The mouse antihuman CD30 antibody AC-10 was radiolabeled with the positron-emitting radionuclide 89Zr. The stability and specificity of 89Zr-desferrioxamine (DFO)-labeled CD30-specific AC-10 antibody (89Zr-DFO-AC-10) was evaluated in vitro. The pharmacokinetics of 89Zr-DFO-AC-10 was studied in BALB/c nude mice bearing subcutaneous human Karpas 299 tumors (CD30-positive model) or A-431 tumors (CD30-negative model) using PET/CT imaging, biodistribution studies, and autoradiography. Results: AC-10 was conjugated with a DFO B chelator and radiolabeled with 89Zr to give formulated 89Zr-DFO-AC-10 with a radiochemical yield of 80%, radiochemical purity greater than 99%, and specific activity of 111–148 MBq/mg. 89Zr-DFO-AC-10 was stable in mouse and human sera and preserved the immunoreactivity toward CD30. Biodistribution data showed the highest tissue accumulation of 89Zr-DFO-AC-10 in CD30-positive tumors, with 37.9% ± 8.2% injected activity per gram of tissue at 72 h after injection, whereas uptake in CD30-negative tumors was 11.0% ± 0.4%. The specificity of 89Zr-DFO-AC-10 binding to CD30 in vivo was confirmed by blocking studies. Time–activity curves showed that between 24 and 144 h after injection, tumor-to-muscle ratios increased from 18.9 to 51.8 in the CD30-positive model and from 4.8 to 8.7 in the CD30-negative model. Tumor-to-blood ratios also increased, from 3.2 to 13.6 and from 1 to 2 in the CD30-positive and -negative models, respectively. Conclusion: Our results demonstrate that for measuring CD30 expression, 89Zr-DFO-AC-10 is a sensitive PET agent with high tumor–to–normal-tissue contrast. 89Zr-DFO-AC-10 is a promising CD30-imaging radiotracer for clinical translation in patients with various lymphomas and other diseases.