RT Journal Article SR Electronic T1 18F-Fluoromisonidazole Quantification of Hypoxia in Human Cancer Patients Using Image-Derived Blood Surrogate Tissue Reference Regions JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 1223 OP 1228 DO 10.2967/jnumed.115.158717 VO 56 IS 8 A1 Mark Muzi A1 Lanell M. Peterson A1 Janet N. O’Sullivan A1 James R. Fink A1 Joseph G. Rajendran A1 Lena J. McLaughlin A1 John P. Muzi A1 David A. Mankoff A1 Kenneth A. Krohn YR 2015 UL http://jnm.snmjournals.org/content/56/8/1223.abstract AB 18F-fluoromisonidazole (18F-FMISO) is the most widely used PET agent for imaging hypoxia, a condition associated with resistance to tumor therapy. 18F-FMISO equilibrates in normoxic tissues but is retained under hypoxic conditions because of reduction and binding to macromolecules. A simple tissue-to-blood (TB) ratio is suitable for quantifying hypoxia. A TB ratio threshold of 1.2 or greater is useful in discriminating the hypoxic volume (HV) of tissue; TBmax is the maximum intensity of the hypoxic region and does not invoke a threshold. Because elimination of blood sampling would simplify clinical use, we tested the validity of using imaging regions as a surrogate for blood sampling. Methods: Patients underwent 20-min 18F-FMISO scanning during the 90- to 140-min interval after injection with venous blood sampling. Two hundred twenty-three 18F-FMISO patient studies had detectable surrogate blood regions in the field of view. Quantitative parameters of hypoxia (TBmax, HV) derived from blood samples were compared with values using surrogate blood regions derived from the heart, aorta, or cerebellum. In a subset of brain cancer patients, parameters from blood samples and from the cerebellum were compared for their ability to independently predict outcome. Results: Vascular regions of heart showed the highest correlation to measured blood activity (R2 = 0.84). For brain studies, cerebellar activity was similarly correlated to blood samples. In brain cancer patients, Kaplan–Meier analysis showed that image-derived reference regions had predictive power nearly identical to parameters derived from blood, thus obviating the need for venous sampling in these patients. Conclusion: Simple static analysis of 18F-FMISO PET captures both the intensity (TBmax) and the spatial extent (HV) of tumor hypoxia. An image-derived region to assess blood activity can be used as a surrogate for blood sampling in quantification of hypoxia.