RT Journal Article
SR Electronic
T1 <strong><sup>99m</sup></strong><strong>Tc-Annexin V</strong><strong> SPECT imaging of femur head avascular necrosis in rabbits: a comparison with <sup>99m</sup>Tc-MDP bone scan</strong>
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1080
OP 1080
VO 56
IS supplement 3
A1 Liu, Yu
A1 Wang, Xiaolong
A1 Wang, Xuemei
A1 Li, Xiao-Feng
A1 Zhao, Jianmin
YR 2015
UL http://jnm.snmjournals.org/content/56/supplement_3/1080.abstract
AB 1080 Objectives To compare early detection effect of apoptosis 99mTc-Annexin V SPECT imaging with 99mTc-MDP bone imaging in a rabbit model of early avascular necrosis of femur head.Methods Adult New Zealand white rabbits were i.m. injected methylprednisolone (7.5 mg/kg) twice per week for up to 8 weeks to generate femur head necrosis model (n=32). Sixteen rabbits were treated with saline as control. 99mTc-Annexin SPECT and 99mTc-MDP SPECT were performed 5, 6, 7 and 8wk after treatment, each point included 8 methylprednisolone treated rabbits and 4 with saline (control). Rabbits were scarified after SPECT studies and bilateral femoral heads were removed and sectioned to detect cells apoptosis by TUNEL.Results Femur head apoptosis cells index was significant higher in 5, 6, 7 and 8 after methylprednisolone treatment (26.82±2.48, 28.73±2.36, 29.54±2.12 and 32.82±2.48, respectively) than saline control (3.51±1.24, 4.13±1.67, 3.78±1.32, 3.49±1.53) (P&lt;0.001). 99mTc-Annexin V uptake in the femur head was significant higher in disease group (T/N: 2.16±0.73, 2.67±0.54, 3.14±0.73 and 3.18±0.72) at each imaging time points than controls (1.34±0.47, 1.42±0.54, 1.28±0.63, 1.43±0.52) (P&lt;0.01 in all). On the other hand, significantly increased in femur head 99mTc-MDP uptake was observed only 8 weeks after methylprednisolone treated rabbits (T/N: 5.49±0.63 vs controls 2.83±0.51)(P&lt;0.01), but not earlier. Furthermore, 99mTc-Annexin V uptake was positively correlated with apoptosis cells index, not 99mTc-MDP.Conclusions 99mTc-Annexin V apoptosis imaging is superior to 99mTc-MDP bone imaging for early detection of glucocorticoid-induced avascular necrosis of femur head in the rabbit. 99mTc-Annexin V apoptosis imaging may early predict glucocorticoid-induced avascular necrosis in clinical setting.Research Support This study was supported by Natural Science foundation of China (81360224)