TY - JOUR T1 - Optimization of non-invasive [<sup>18</sup>F]fluoromethyl-PBR28 PET study for quantification of neuroinflammation using alternative reference compartments in rat Parkinson’s disease model JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1756 LP - 1756 VL - 56 IS - supplement 3 AU - Hyun Soo Park AU - Byung Seok Moon AU - Jae Ho Jung AU - Byung Chul Lee AU - Sang Eun Kim Y1 - 2015/05/01 UR - http://jnm.snmjournals.org/content/56/supplement_3/1756.abstract N2 - 1756 Objectives The absence of the reference tissue causes substantial inefficiency in the preclinical stage of the PET radioligand development for neuroinflammation imaging. We performed [18F]fluoromethyl-PBR28 PET studies to identify a suitable alternative reference compartment for simplifying the data acquisition and the quantification of the neuroinflammation in rat Parkinson’s disease (PD) model.Methods LPS-induced PD rat models were underwent dynamic [18F]fluoromethyl-PBR28 PET studies. The image-derived input function (IDIF) using the left ventricle (LV) and contralateral side of LPS-infusion in the striatum were selected as alternative reference compartments. Distribution volume (DV) and binding potential (BPND) were estimated for each reference compartment using 2-tissue compartment model and non-invasive graphical analysis, respectively. Time-activity curves were rebinned and target to reference compartment concentration ratio (CR) of each binning were calculated. The correlation between DV, BPND and CR were statistically tested.Results DV (5.5±1.6) and BPND (2.8±0.9) of [18F]fluoromethyl-PBR28 in the ipsilateral striatum of LPS-infusion were significantly correlated (R2=0.970, P&lt;0.05). A significant correlation between CR of 40-60 min binning of the time-activity curves and BPND was found (R2=0.997, P&lt;0.05).Conclusions Data demonstrated that contralateral side of LPS-infusion in the striatum in PD rat model is an alternative reference compartment for quantification of neuroinflammation using non-invasive graphical analysis for [18F]fluoromethyl-PBR28 PET studies. And semi-quantification of 20 min-static image data acquired 40 min after the injection provides reliable results represent BPND of [18F]fluoromethyl-PBR28. ER -