RT Journal Article
SR Electronic
T1 Monitoring cholesterol transport in vivo by PET
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 466
OP 466
VO 56
IS supplement 3
A1 Belov, Vasily
A1 Mushti, Chandra
A1 Levine, Dylan
A1 Papisov, Mikhail
A1 Fischman, Alan
YR 2015
UL http://jnm.snmjournals.org/content/56/supplement_3/466.abstract
AB 466 Objectives Incidences of diseases characterized by alterations in cholesterol homeostasis (e.g., atherosclerosis, obesity, diabetes) are increasing. Thus, there is a growing need for methods for comprehensive quantitative evaluation of such alterations. The data reported previously are not sufficiently detailed due to the limited capabilities of early imaging modalities. In a present study, we took advantage of the superior spatial and time resolution of PET to develop an imaging method for quantitative real-time monitoring of the transport of cholesterol in vivo.Methods 19-Iodocholesterol (IC) was synthetized following the published procedures and radiolabeled with 124I by isotope exchange with the radiochemical yield and purities of 58% and 75%, respectively. Biokinetics of [124I] was studied by PET in six SD rats (~250 g). To this end, [124I]IC was incorporated in the lipidic component of the rat plasma and administered IV. Dynamic and static images were acquired for 4 days using microPET Focus 220 imager (Siemens) interfaced with CereTom NL 3000 CT scanner (Neurologica). Time dependences of organ uptake were quantified by ROI analysis.Results Initial de-halogenation and labile 124I content did not exceed 5% of the injected dose (%ID) as quantified by total [124I]IC accumulation in the thyroid, bladder, and stomach walls. Liver was the organ of the greatest uptake over the entire period of observation. Other, minor sites of [124I]IC uptake included: kidneys, spleen, pancreas, large and small intestines, caecum, lungs, muscle, and fat. Progressive accumulation of [124I]IC in the adrenal gland at up to 0.7%ID has been characteristic and was consistent with the reported behavior of cholesterol in vivo.Conclusions [124I]IC is a stable probe suitable for long-term imaging of cholesterol transport in vivo by PET and quantification of the kinetic manifestations of the alterations in cholesterol homeostasis.Research Support NIH