RT Journal Article SR Electronic T1 Cerenkov Luminescence Imaging for Radiation Dose Calculation of a 90Y-Labeled Gastrin-Releasing Peptide Receptor Antagonist JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 805 OP 811 DO 10.2967/jnumed.114.149054 VO 56 IS 5 A1 Christian Lohrmann A1 Hanwen Zhang A1 Daniel L.J. Thorek A1 Pooja Desai A1 Pat B. Zanzonico A1 Joseph O’Donoghue A1 Christopher P. Irwin A1 Thomas Reiner A1 Jan Grimm A1 Wolfgang A. Weber YR 2015 UL http://jnm.snmjournals.org/content/56/5/805.abstract AB 90Y has been used to label various new therapeutic radiopharmaceuticals. However, measuring the radiation dose delivered by 90Y is challenging because of the absence of suitable γ emissions and its low abundance of positron emissions. For the treatment of prostate cancer, radiolabeled gastrin-releasing peptide receptor (GRPr) antagonists have yielded promising results in mouse models. In this study, we evaluated whether Cerenkov luminescence imaging (CLI) could be used to determine radiation doses of a 90Y-labeled GRPr antagonist in nude mice. Methods: Mice bearing subcutaneous prostate cancer xenografts were injected with 0.74–18.5 MBq of the 90Y-labeled GRPr antagonist DOTA-AR and underwent in vivo and ex vivo CLI at 1–48 h after injection. After imaging, animals were sacrificed, their tumors and organs were harvested, and the activity concentration was measured by liquid scintillation counting. In a second set of experiments, Cerenkov photon counts for tumor and kidney on in vivo CLI were converted to activity concentrations using conversion factors determined from the first set of experiments. Results: 90Y-DOTA-AR concentration in the 3 tumor models ranged from 0.5% to 4.8% of the injected activity per gram at 1 h after injection and decreased to 0.05%–0.15 injected activity per gram by 48 h after injection. A positive correlation was found between tumor activity concentrations and in vivo CLI signal (r2 = 0.94). A similar correlation was found for the renal activity concentration and in vivo Cerenkov luminescence (r2 = 0.98). Other organs were not distinctly visualized on the in vivo images, but ex vivo CLI was also correlated with the radioactivity concentration (r2 = 0.35–0.94). Using the time–activity curves from the second experiment, we calculated radiation doses to tumor and kidney of 0.33 ± 0.12 (range, 0.21–0.66) and 0.06 ± 0.01 (range, 0.05–0.08) Gy/MBq, respectively. Conclusion: CLI is a promising, low-cost modality to measure individual radiation doses of 90Y-labeled compounds noninvasively. The use of Cerenkov imaging is expected to facilitate the development and comparison of 90Y-labeled compounds for targeted radiotherapy.