RT Journal Article
SR Electronic
T1 The 18-kDa Mitochondrial Translocator Protein in Human Gliomas: An <sup>11</sup>C-(<em>R</em>)PK11195 PET Imaging and Neuropathology Study
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 512
OP 517
DO 10.2967/jnumed.114.151621
VO 56
IS 4
A1 Zhangjie Su
A1 Federico Roncaroli
A1 Pascal F. Durrenberger
A1 David J. Coope
A1 Konstantina Karabatsou
A1 Rainer Hinz
A1 Gerard Thompson
A1 Federico E. Turkheimer
A1 Karolina Janczar
A1 Daniel Du Plessis
A1 Andrew Brodbelt
A1 Alan Jackson
A1 Alexander Gerhard
A1 Karl Herholz
YR 2015
UL http://jnm.snmjournals.org/content/56/4/512.abstract
AB The 18-kDa mitochondrial translocator protein (TSPO) is upregulated in high-grade astrocytomas and can be imaged by PET using the selective radiotracer 11C-(R)PK11195. We investigated 11C-(R)PK11195 binding in human gliomas and its relationship with TSPO expression in tumor tissue and glioma-associated microglia/macrophages (GAMs) within the tumors. Methods: Twenty-two glioma patients underwent dynamic 11C-(R)PK11195 PET scans and perfusion MR imaging acquisition. Parametric maps of 11C-(R)PK11195 binding potential (BPND) were generated. Coregistered MR/PET images were used to guide tumor biopsy. The tumor tissue was quantitatively assessed for TSPO expression and infiltration of GAMs using immunohistochemistry and double immunofluorescence. The imaging and histopathologic parameters were compared among different histotypes and grades and correlated with each other. Results: BPND of 11C-(R)PK11195 in high-grade gliomas was significantly higher than in low-grade astrocytomas and low-grade oligodendrogliomas. TSPO in gliomas was expressed predominantly by neoplastic cells, and its expression correlated positively with BPND in the tumors. GAMs only partially contributed to the overall TSPO expression within the tumors, and TSPO expression in GAMs did not correlate with tumor BPND. Conclusion: PET with 11C-(R)PK11195 in human gliomas predominantly reflects TSPO expression in tumor cells. It therefore has the potential to effectively stratify patients who are suitable for TSPO-targeted treatment.