PT  - JOURNAL ARTICLE
AU  - Charles Zhu
AU  - Amey Bandekar
AU  - Sangeeta Ray
AU  - Martin Pomper
AU  - Frank Bruchertseifer
AU  - Alfred Morgenstern
AU  - Stavroula Sofou
TI  - Anti-PSMA labeled liposomes loaded with Actinium-225 for potential antivascular alpha-radiotherapy
DP  - 2014 May 01
TA  - Journal of Nuclear Medicine
PG  - 640--640
VI  - 55
IP  - supplement 1
4099  - http://jnm.snmjournals.org/content/55/supplement_1/640.short
4100  - http://jnm.snmjournals.org/content/55/supplement_1/640.full
SO  - J Nucl Med2014 May 01; 55
AB  - 640 Objectives We studied targeted liposomes loaded with the alpha-particle generator actinium-225 (225Ac) to kill cells expressing the prostate-specific-membrane-antigen (PSMA) with the overall goal of evaluating the potential of these liposomes for targeted antivascular alpha-radiotherapy. The targeting moiety is designed to bind PSMA, which is unique to human neovasculature for a variety of solid tumors. The therapeutic radionuclide 225Ac emits a total of four alpha-particles per decay, providing highly lethal and localized irradiation of targeted cells with minimal exposure of surrounding healthy tissues. Finally, the delivery vehicle, a liposome, can be loaded with substantial levels of radioactivity and can be engineered for rapid clearance to shift the distribution away from the kidneys, the dose-limiting organ, and toward the spleen and liver. Methods Liposomes were labeled with two types of PSMA-targeting ligands: a fully human PSMA antibody (mAb), and a urea-based low-molecular-weight agent. Targeting selectivity, extent of internalization and killing efficacy were evaluated on monolayers of LNCaP cells intrinsically expressing PSMA and on monolayers of HUVEC induced to express PSMA in static incubation conditions and under flow at a 15s-1 shear rate in a parallel-plate flow chamber. Results Specific cell association increases with incubation time and decreases by twofold upon introduction of flow. Both types of radiolabeled PSMA-targeting liposomes exhibited similar killing efficacy, which was greater than the efficacy of the radiolabeled control mAb. Fluorescence confocal microscopy demonstrated that targeted liposomes localized near the nucleus, unlike antibodies which localized near the plasma membrane, suggesting that perinuclear localization may enhance the probability of alpha-particle trajectories crossing the nuclei. Conclusions These findings suggest the potential of PSMA-targeting liposomes. Research Support Supported by the American Cancer Society Grant RSG-12-044-01 and in part by the National Science Foundation Grant DMR1207022 and the Charles and Johanna Busch Memorial Fund.