RT Journal Article
SR Electronic
T1 Separation of TcO<sub>4</sub><sup>-</sup> and MoO<sub>4</sub><sup>2-</sup> using a PEG coated C18 SPE cartridge
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1001
OP 1001
VO 54
IS supplement 2
A1 Andersson, Jan
A1 Wilson, John
A1 Gagnon, Katherine
A1 Romaniuk, Joseph
A1 McEwan, Alexander
A1 Abrams, Douglas
A1 McQuarrie, Steve
YR 2013
UL http://jnm.snmjournals.org/content/54/supplement_2/1001.abstract
AB 1001 Objectives A critical aspect in the feasibility of routine cyclotron production of 99mTc via the 100Mo(p,2n)99mTc reaction is the separation of 99mTc from the bulk 100Mo target. The aim of this work was to assess if polyethylene glycol (PEG) coated on commercially available solid phase extraction (SPE) cartridges can be a viable method for the separation of pertechnetate (TcO4-) from molybdate (MoO42-). The recovered [99mTc]TcO4- purity and the recovered MoO42- yield for possible recycling was evaluated. Methods Separations were performed on an automated chemistry unit. C18 cartridges containing 3 g of resin were coated by pouring PEG3000 solution through the cartridge and then washing with deionized water (D.I. H2O). PEG concentration in the final product was evaluated by iodine visualization TLC. Initial separation testing was performed using generator eluted [99mTc]TcO4- and [99Mo]MoO42- in a loading solution containing 300 mg Mo powder dissolved in 10 mL H2O2 and 10 mL (NH4)2CO3 (3 M). After loading, the column was washed with 5 mL (NH4)2CO3 (1 M) followed by 1.5 mL D.I. H2O. The [99mTc]TcO4- was eluted using 10 mL D.I. H2O and passed through a strong cation exchange column and an alumina column. The [99mTc]TcO4- was eluted from the alumina column using 10 mL of isotonic saline. Results Recovery of 99mTc was 92 ± 4 % (n=4). Greater than 99.5 % of the 99Mo was present in the load/wash fractions (n=2) thus allowing for minimal losses in terms of recycling enriched 100Mo. PEG3000 concentration in the final product was lower than the limit of detection for our TLC spot test (0.1 mg/mL), pH was 6 and the product was clear and colorless Conclusions We developed a practical and easily accessible system for separation of 99mTc-pertechnetate, with good recovery yields for [99mTc]TcO4- and promising features for molybdenum recovery. The final product passed appropriate QC tests. Research Support This work was supported by research grants from NSERC/CIHR and Natural Resources Canada.