RT Journal Article
SR Electronic
T1 Mice strain differences in small animal PET [11C]-PiB and [18F]-Florbetaben imaging of β-amyloid plaques
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1764
OP 1764
VO 54
IS supplement 2
A1 Waldron, Ann-Marie
A1 Verhaeghe, Jeroen
A1 Wyffels, Leonie
A1 Bigot, Christian
A1 Schmidt, Mark
A1 Langlois, Xavier
A1 Van Der Linden, Annemie
A1 Stroobants, Sigrid
A1 Staelens, Steven
YR 2013
UL http://jnm.snmjournals.org/content/54/supplement_2/1764.abstract
AB 1764 Objectives To investigate the contribution of strain differences on the β-amyloid specific [11C]-PiB and [18F]-Florbetaben ([18F]-FBB) signal in small animal PET. Methods Studies were performed on 22-25mth BRI1-42 (n=6) and APPPS1-21 (n=6) mice, and their respective littermate controls (C57B/6J n=7, B6C3 n=6). To evaluate β-amyloid burden, mice from each strain underwent; a) 60min dynamic scans after an i.v bolus of [11C]-PiB (8.8±0.56MBq, S.A 90.7±1.2 GBq/μmol), and b) 15 or 25min static scans following an i.v injection of [18F]-FBB and an uptake period of 35min (7.5±0.34MBq and 3.9±0.18MBq for APPPS1-21 and BRI1-42 mice respectively, S.A 127.5 ± 67.2 GBq/ μmol). PET data was reconstructed using OSEM2D (4 subsets, 16 iterations). The data was not corrected for attenuation and scatter.Relevant VOI’s were delineated through registration of PET/CT images with a predefined mouse brain VOI template and tracer uptake was quantified through standardized uptake value (SUV). Results Both [11C]-PiB and [18F]-FBB demonstrated significant uptake in APPPS1-21 mice with the highest significance revealed in the hippocampus (p=0.0002 and p<0.0001 for [11C]-PiB and [18F]-FBB respectively) and additionally in the striatum (p<0.0001) for [18F]-FBB. In the same cohort of mice, [18F]-FBB failed to identify significant uptake in the cerebellum and olfactory bulb, regions showing significant uptake with [11C]-PiB. In BRI1-42 mice both [11C]-PiB and [18F]-FBB failed to demonstrate specific tracer accumulation. Conclusions The strain investigated is of paramount importance to successful β-amyloid imaging.