@article {Kang1330, author = {Lei Kang and Xiaojie Xu and Rong Fu Wang and Ping Yan and Chunli Zhang}, title = {Evaluation of the effect of microRNA on hepatocellular carcinoma proliferation and metastasis by FDG small-animal PET}, volume = {54}, number = {supplement 2}, pages = {1330--1330}, year = {2013}, publisher = {Society of Nuclear Medicine}, abstract = {1330 Objectives The objective of this study is to investigate the value of 18FDG PET in assessing the role of miR-X in hepatocellular carcinoma (HCC) xenograft and metastasis models. Methods After the stably overexpressed miR-X HCC cell lines (HepG2 and MHCC97H) were constructed by lentiviral vector transfection, in vitro studies were performed evaluating the ability of miR-X in FDG uptake, tumor proliferation and metastasis by colony formation and Matrigel invasion assay. Moreover, in vivo small-animal PET and biodistribution studies were performed in HepG2 xenografts or MHCC97H metastasis models. Besides, the expression of markers in insulin-like growth factor-1 receptor (IGF-1R)/ phosphatidyl- inositol-3-kinase (PI3K)/AKT pathways and glucose metabolism in HCC cell lines and tumor xenografts were evaluated by Western blot assay. Results The proliferated and invaded abilities were inhibited in miR-148 overexpressed HCC cells in vitro, as well as the FDG cellular uptake. The inhibited FDG uptake by miR-X in HCC cells was correlated with the suppression of IGF-1R/PI3K/AKT pathway and GLUT1 protein. For HepG2 tumor xenografts, small-animal PET clearly showed relatively low FDG accumulation in the miR-X group, which was verified by the suppressed FDG uptake and IGF-1R/PI3K/AKT pathway of ablated tumors. Quantitative analysis showed that the tumor-to-muscle ratios of maximum counts decreased significantly in the miR-X overexpressed tumors, especially in the PET delayed images. For MHCC97H metastasis model, the FDG PET images of living mice and the ablated organs discovered less metastatic nodules in livers and lungs in the miR-X group, which were consistent with anatomy. Conclusions 18FDG small-animal PET is useful for evaluating the negative role of miR-X in HCC living models. MiR-X may suppress the FDG uptake in HCC via the inhibition of IGF-1R/PI3K/AKT pathway in glucose metabolism.}, issn = {0161-5505}, URL = {https://jnm.snmjournals.org/content/54/supplement_2/1330}, eprint = {https://jnm.snmjournals.org/content}, journal = {Journal of Nuclear Medicine} }