RT Journal Article
SR Electronic
T1 Long-term evaluation of TiO2-based 68Ge/68Ga generators and optimized automation of 68Ga-DOTATOC radiosynthesis
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1457
OP 1457
VO 53
IS supplement 1
A1 Lin, Mai
A1 Ranganathan, David
A1 Lapi, Suzanne
A1 Welch, Michael
YR 2012
UL http://jnm.snmjournals.org/content/53/supplement_1/1457.abstract
AB 1457 Objectives Interest in using 68Ga (t1/2: 68 min; β+%: 89%; Eβ+max: 1.92 MeV) is rapidly increasing for clinical PET applications due to its favorable imaging characteristics and accessibility. The focus of this study was to provide our long-term evaluations of the two TiO2-based 68Ge/68Ga generators and develop an optimized automation strategy to synthesize 68Ga-DOTATOC by using HEPES as the buffer system. Methods Characteristics of the two TiO2-based generators were evaluated. The elution profiles of the generators were monitored by using 0.1 N HCl as the eluent. Fractionation and purification steps using a cation-exchange column were performed. The purified 68Ga-eluate was used for testing and optimizing automation of 68Ga-DOTATOC radiosynthesis. Results Both generator systems were able to produce 68Ga with consistent elution profiles. While there was no significant difference in the 68Ge breakthrough in each collected fraction from both generators at early time points, the breakthrough in the 1st generator was significantly higher than that of the 2nd generator after 200 days use. In addition, the breakthrough components could be reduced by purifying the eluate with a Strata X-C column. By applying the purified 68Ga-eluate to our automated module, we were able to obtain 68Ga-DOTATOC with high specific activity (45.88 GBq/µmol) and radiochemical purity (> 99%) with a synthesis time of 15 min. Conclusions We have characterized two TiO2-based 68Ge/68Ga generators and developed optimized automated approach by using HEPES as the buffer system. These data will be useful in standardizing the evaluation of 68Ge/68Ga generators and automation strategies to comply with regulatory issues for clinical use. Research Support This work was supported by DOE Integrated Research Training Program of Excellence in Radiochemistry (DE-SC0002032)