RT Journal Article
SR Electronic
T1 Injection with 177Lu-octreotate reveals distinct gene expression response in kidney tissue
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1190
OP 1190
VO 53
IS supplement 1
A1 Schüler, Emil
A1 Parris, Toshima
A1 Rudqvist, Nils
A1 Helou, Khalil
A1 Forssell-Aronsson, Eva
YR 2012
UL http://jnm.snmjournals.org/content/53/supplement_1/1190.abstract
AB 1190 Objectives The purpose of this study was to investigate transcriptional changes in mouse kidney in response to i.v. injection of 177Lu-octreotate. Methods Female Balb/c mice were i.v. injected with 0, 1.3, 3.6, 14, 45, or 140 MBq 177Lu-octreotate. The animals were sacrificed and the kidneys surgically removed 24h after injection. Total RNA was extracted from the kidney cortex and medulla separately. The absorbed doses to the kidneys were calculated according to the MIRD formalism and ranged between 0.13 and 13 Gy. Illumina MouseRef-8 Whole-Genome Expression Beadchips were used to identify differentially expressed transcripts between irradiated and non-irradiated tissue. Results A clear transcriptional response was found in the kidney cortex and medulla following irradiation. The number of regulated transcripts differed in the two tissues (281 vs 480 differentially expressed transcripts). The highest number of regulated transcripts was found at 45 MBq IA in kidney cortex and at 14 MBq IA in kidney medulla. Five biological processes were shared by all dose levels in kidney cortex (immune response, amino acid transport, circadian rhythm, rhythmic processes, and regulation of lipoprotein lipase activity) and three processes in the medulla (glycerol metabolism, immune response, and defense response). Among all biological processes affected in the two tissues, metabolic processes were the most pronounced. However, immune response was the only biological process which was affected in all dose levels for both tissues. Conclusions In this study the early response of 177Lu-octreotate on kidney tissues was investigated. The results indicate that at this early time point, metabolic processes are primarily affected. Clear differences in response between irradiated kidney cortex and medulla were primarily found in cellular and system processes