PT  - JOURNAL ARTICLE
AU  - Mori, Tetsuya
AU  - Takaki, Kenji
AU  - Kiyono, Yasushi
AU  - Fujibayashi, Yasuhisa
AU  - Okazawa, Hidehiko
TI  - Development of an automatic Copper-62 ATSM preparation module in aseptic condition
DP  - 2012 May 01
TA  - Journal of Nuclear Medicine
PG  - 1488--1488
VI  - 53
IP  - supplement 1
4099  - http://jnm.snmjournals.org/content/53/supplement_1/1488.short
4100  - http://jnm.snmjournals.org/content/53/supplement_1/1488.full
SO  - J Nucl Med2012 May 01; 53
AB  - 1488 Objectives Copper-62 labeled diacetyl-bis(N4-methylthiosemicarbazone), 62Cu-ATSM, is one of promising PET radiopharmaceuticals for hypoxia imaging in clinical trial. The 62Cu can obtained from 62Zn/62Cu generator with a glycine solution and it is possible to try PET studies without a cyclotron. We have designed and developed the flexible module system using miniature humanoid robot kits which are commercially available as hobby. The system was designed a three-way valve unit, a syringe unit and I/O units for controlling external devises. These units were adjusted a sterilized disposable three-way valve and syringe, to realize sterility as well as disposability. In addition, this concept allowed us to prevent cross-contamination of the reagents. In this study, we assembled an automatic module for 62Cu-ATSM preparation in aseptic condition. Methods The 62Zn/62Cu generator was obtained from National Institute of Radiological Sciences. The automatic module was consisted 2 syringe units and 6 valve units. A glycine solution (200 mM, 5 mL) was used for 62Cu elution from the 62Zn/62Cu generator and then added 5 mL of saline solution including 50 mg of sodium ascorbate to prevent decomposition before injection. Results We assessed 10 generators for the 62Cu-ATSM preparations. The preparation time was accomplished at sufficient reproducibility under pyrogen-free condition in less than 1 min. The quality control such as radiochemical purity, pH, and sterility were passed. The results were similar to that of manual operations. Conclusions The automation of the 62Cu-ATSM preparation was produced high quality radiopharmaceutical. In addition, it reduced a human error and an exposure module. Thus, the convenient system may help the research of the PET probe development