RT Journal Article
SR Electronic
T1 99mTc labeled doxorubicin as a potential radioligand for the early cancer detection and treatment response monitoring
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1531
OP 1531
VO 51
IS supplement 2
A1 Singh, Baljinder
A1 Kumar, Pardeep
A1 Sharma, Sarika
A1 Mishra, Anil
A1 Mittal, Bhagwant
YR 2010
UL http://jnm.snmjournals.org/content/51/supplement_2/1531.abstract
AB 1531 Objectives To characterize the radiolabeled doxorubcin and its scintigraphic evaluation for the detection of cancer. Methods Doxorubicin salt was dissolved in sterile water & Sncl2.2H2O was used as reducing agent. Required radioactivity of 99mTc was added in the sterile water. pH was adjusted to 6-7. Blood kinetics and distribution studies were performed in mice.Tumours were induced experimentally in mice by using Hershly acitis tumor cell lines.briefly 0.5 mci radioactivity of 99mTc-doxorubicin was injected i.v. into each animal in the tail vein & images (isotime images) were acquired at the different time intervals of 1.5hr, 3hr and 4hr in 256x256 matrix (5min acquisition) on single head gamma camera. Results Labeling efficiency of the radioligand was observed to be ≥ 95.0%. The protein binding was about 88%. The preparation remained stable till 24h.The radiochemical purity was ≥98.0%. Blood kinetic data suggested that approximately 0.97% of activity of the radio label remained in circulation at 24h.The scintigraphic data in tumor bearing mice indicated a consistently rising lesion to background ratio of the radio tracer till 4h. Conclusions The labeled anticancer drug can be used as a tool for the early detection of cancer and the localization of the radiolabeled doxorubicin in the tumor will indicate a good response of this agent and therefore help in the prediction of treatment response. This scintigraphic approach, therefore, could be a powerful tool for the primary cancer screening in precancerous and early cancerous stage, as opposed to the existing scintigraphic and radiological modalities which are mainly focused towards the detection and screening of secondaries and not the primaries. This technique however, needs further validation through animal experimentation and clinical studies. Research Support Life Science Research Board, DRDO, Ministry of Defense, Govt.of India