TY - JOUR T1 - Comparison of the tumor and inflammation uptake of [<sup>18</sup>F]-deoxy-1-fluoro-<em>scyllo</em>-inositol and [<sup>18</sup>F]-FDG in athymic mice bearing human breast cancer xenografts JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1515 LP - 1515 VL - 51 IS - supplement 2 AU - Kristin McLarty AU - Matthew Moran AU - Deborah Scollard AU - Conrad Chan AU - JoAnne McLaurin AU - Mark Nitz AU - Alan Wilson AU - Sylvain Houle AU - Raymond Reilly AU - Neil Vasdev Y1 - 2010/05/01 UR - http://jnm.snmjournals.org/content/51/supplement_2/1515.abstract N2 - 1515 Objectives Our preliminary work demonstrated that [18F]1-deoxy-1-fluoro-scyllo-inositol ([18F]-SI) may be a promising PET imaging agent for breast cancer (BC) (Chem. Commun., 2009, 5527). The objectives of the present work were to automate the radiosynthesis of [18F]-SI and to compare the uptake of [18F]-SI and [18F]-FDG in human BC xenografts and inflammation in athymic mice. Methods Radiosynthesis of [18F]-SI was automated, as per our previously described 3-step reaction, using a commercial synthesis module. Subcutaneous MDA-MB-231 human BC xenografts and turpentine-induced s.c. inflammation were established in female CD1 athymic mice. Mice (n=5 per group) were intravenously injected with [18F]-SI or [18F]-FDG (7.5 ± 0.6 MBq) and immediately anesthetized using 2% isoflurane in oxygen. PET-CT imaging and ex vivo biodistribution studies were performed at 60 minutes post-injection. Results [18F]-SI was prepared with good radiochemical yields (23.8 ± 0.6%; uncorrected), high specific activity (&gt;10 Ci/μmol at EOS; determined by LC-MS-MS) and high radiochemical purity (&gt;99%) within 65 minutes. The tumor uptake of [18F]-SI was comparable to that of [18F]-FDG (4.6 ± 0.5 %ID/g vs. 5.5 ± 2.1 %ID/g, P=0.40. Uptake of [18F]-SI in inflammatory tissue was moderately lower than that of [18F]-FDG (3.5 ± 0.5 %ID/g vs. 5.5 ± 1.0 %ID/g, P=0.004). All tumors and inflammation were visualized by PET imaging and there was a reasonable correlation between the biodistribution and ROI analyses (r=0.70). Consistent with our preliminary results, brain uptake of [18F]-SI was negligible compared to that of [18F]-FDG (0.5 ± 0.1 %ID/g vs. 8.1 ± 1.0 %ID/g, P&lt;0.0001). Conclusions Radiosynthesis of [18F]-SI was successfully automated for routine production. Uptake of [18F]-SI in tumors and inflammation was comparable to that of [18F]-FDG. Future studies evaluating [18F]-SI for visualizing different BC xenograft phenotypes are underway. Research Support OICR, NSERC and CIH ER -