RT Journal Article
SR Electronic
T1 Comparison of the tumor and inflammation uptake of [<sup>18</sup>F]-deoxy-1-fluoro-<em>scyllo</em>-inositol and [<sup>18</sup>F]-FDG in athymic mice bearing human breast cancer xenografts
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1515
OP 1515
VO 51
IS supplement 2
A1 McLarty, Kristin
A1 Moran, Matthew
A1 Scollard, Deborah
A1 Chan, Conrad
A1 McLaurin, JoAnne
A1 Nitz, Mark
A1 Wilson, Alan
A1 Houle, Sylvain
A1 Reilly, Raymond
A1 Vasdev, Neil
YR 2010
UL http://jnm.snmjournals.org/content/51/supplement_2/1515.abstract
AB 1515 Objectives Our preliminary work demonstrated that [18F]1-deoxy-1-fluoro-scyllo-inositol ([18F]-SI) may be a promising PET imaging agent for breast cancer (BC) (Chem. Commun., 2009, 5527). The objectives of the present work were to automate the radiosynthesis of [18F]-SI and to compare the uptake of [18F]-SI and [18F]-FDG in human BC xenografts and inflammation in athymic mice. Methods Radiosynthesis of [18F]-SI was automated, as per our previously described 3-step reaction, using a commercial synthesis module. Subcutaneous MDA-MB-231 human BC xenografts and turpentine-induced s.c. inflammation were established in female CD1 athymic mice. Mice (n=5 per group) were intravenously injected with [18F]-SI or [18F]-FDG (7.5 ± 0.6 MBq) and immediately anesthetized using 2% isoflurane in oxygen. PET-CT imaging and ex vivo biodistribution studies were performed at 60 minutes post-injection. Results [18F]-SI was prepared with good radiochemical yields (23.8 ± 0.6%; uncorrected), high specific activity (&gt;10 Ci/μmol at EOS; determined by LC-MS-MS) and high radiochemical purity (&gt;99%) within 65 minutes. The tumor uptake of [18F]-SI was comparable to that of [18F]-FDG (4.6 ± 0.5 %ID/g vs. 5.5 ± 2.1 %ID/g, P=0.40. Uptake of [18F]-SI in inflammatory tissue was moderately lower than that of [18F]-FDG (3.5 ± 0.5 %ID/g vs. 5.5 ± 1.0 %ID/g, P=0.004). All tumors and inflammation were visualized by PET imaging and there was a reasonable correlation between the biodistribution and ROI analyses (r=0.70). Consistent with our preliminary results, brain uptake of [18F]-SI was negligible compared to that of [18F]-FDG (0.5 ± 0.1 %ID/g vs. 8.1 ± 1.0 %ID/g, P&lt;0.0001). Conclusions Radiosynthesis of [18F]-SI was successfully automated for routine production. Uptake of [18F]-SI in tumors and inflammation was comparable to that of [18F]-FDG. Future studies evaluating [18F]-SI for visualizing different BC xenograft phenotypes are underway. Research Support OICR, NSERC and CIH