@article {Yoon1774, author = {Joon-Kee Yoon and Bok-Nam Park and Wooyoung Shim and Young Hwan Ahn and Gwang Lee}, title = {Transient cell cycle arrest of rat bone marrow mesenchymal stem cells by In-111 labeling}, volume = {51}, number = {supplement 2}, pages = {1774--1774}, year = {2010}, publisher = {Society of Nuclear Medicine}, abstract = {1774 Objectives The aim of this study was to evaluate the effect of In-111 labeling on the viability and functions and BMSCs. Methods Rat BMSCs were labeled with various doses of In-111 tropolone. Growth curves, FACS analysis after staining with BrdU (5-bromo-2-deoxyuridine), and microscopic evaluation after X-gal (5-bromo-4-chloro-3-indolyl-D-galactopyranoside) staining were performed for each dose (0.4-11.1 Bq/cell) until 14th day after labeling. For higher doses of In-111 (11.1 and 33.3 Bq/cell), FACS analysis after staining with Annexin V-FITC and PI (propidium iodide) was performed at early (3 and 12 hr) and late (7 days) stages. Results For lower doses of In-111 (0.4 and 1.1 Bq/cell), the growth of labeled stem cells was not different from that of control. However, the labeling with higher doses of In-111 (4.4 and 11.1 Bq/cell) inhibited the proliferation of BMSCs significantly from the 3rd day to 14th day (figure 1). FACS analysis also revealed less BrdU positive cells in BMSCs at concentrations of 0.1, 4.4 and 11.1 Bq/cell at 3rd day after labeling. Of these, the number of BrdU positive cells BMSCs labeled with In-111 of 1.1 and 4.4 Bq/cell was restored later. On the contrary, BMSCs labeled with 11.1 Bq In-111/cell could not recover from cell cycle arrest (figure 2). X-gal staining was not prominent in all concentrations until 14th day after labeling. FACS analysis with Annexin V-FITC and PI also revealed no significant apoptosis or necrosis in both early and late stages. Conclusions We observed the dose-dependent growth inhibition of BMSCs by In-111 labeling, which was developed by dose-dependent, transient cell cycle arrest, not by cellular senescence or apoptosis/necrosis}, issn = {0161-5505}, URL = {https://jnm.snmjournals.org/content/51/supplement_2/1774}, eprint = {https://jnm.snmjournals.org/content}, journal = {Journal of Nuclear Medicine} }