RT Journal Article
SR Electronic
T1 Accumulation and transport mechanism of <em>anti</em>-1-amino-3-[<sup>18</sup>F]fluorocyclobutane-1-carboxylic acid in representative human carcinomas
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1512
OP 1512
VO 51
IS supplement 2
A1 Kobayashi, Masato
A1 Kagawa, Shinya
A1 Nishii, Ryuichi
A1 Takahashi, Fumiya
A1 Ohe, Kazuyo
A1 Yoshimoto, Mitsuyoshi
A1 Shikano, Naoto
A1 Higashi, Tatsuya
A1 Nagamachi, Shigeki
A1 Kawai, Keiichi
YR 2010
UL http://jnm.snmjournals.org/content/51/supplement_2/1512.abstract
AB 1512 Objectives Anti-1-amino-3-[18F]fluorocyclobutane-1-carboxylic acid ([18F]FACBC) is a promising radiotracer for in-vivo imaging human glioblastoma and prostate carcinoma. However, there are no reports for detecting other carcinomas using [18F]FACBC. We examined accumulation and transport mechanism of [18F]FACBC in representative human-derived carcinoma cells and compared with [18F]FDG. Methods The [1-14C]FACBC or [14C]FDG were added in five types of cultured human carcinoma cells (epidermal carcinoma: A431, colorectal carcinoma: LS180, lung carcinoma: PC14/GL, H441/GL, breast carcinoma: MDA-MB435) with and without a specific Na+-dependent system A inhibitor (MeAIB) and Na+-independent system L inhibitor (BCH). The radioactivity in the cells was measured at incubation time of 0.5, 1, 3, 10 min. The accumulation of [1-14C]FACBC was compared with that of [14C]FDG. The transport mechanism of [1-14C]FACBC was investigated using gene analysis of DNA microarray and real-time polymerase chain reaction method. Results The accumulation of [1-14C]FACBC in the A431 and PC14 gradually increased over time, while the LS180, H441 and MDA-MB435 reached peak at incubation time of 3 min and then decreased. From results of gene analysis, the main transport in the A431 and PC14 was through both system A and system L, and the LS180, H441 and MDA-MB435 was through only system L. On the other hand, the accumulation of [14C]FDG lineally increased in all carcinoma cells. The values of [1-14C]FACBC at 3 min was 1.6-4.7 times higher than those of [14C]FDG. Conclusions [18F]FACBC will be useful radiotracer for diagnosing the human carcinomas by means of accumulation change depending on expression level of system A and/or system L in the carcinomas even if there are little differences of [18F]FDG accumulation in the carcinomas