PT  - JOURNAL ARTICLE
AU  - Son Joo  Ahn
AU  - Yong Hyun Jeon
AU  - Sang-Woo  Lee
AU  - Byeong-Cheol  Ahn
AU  - Jeoung-Hee  Ha
AU  - Jae-Tae  Lee
TI  - The change of F-18 FDG and H-3 PCV uptakes after transduction of micro RNA for hexokinase II and HSV1-sr39 gene in cancer cells
DP  - 2009 May 01
TA  - Journal of Nuclear Medicine
PG  - 1604--1604
VI  - 50
IP  - supplement 2
4099  - http://jnm.snmjournals.org/content/50/supplement_2/1604.short
4100  - http://jnm.snmjournals.org/content/50/supplement_2/1604.full
SO  - J Nucl Med2009 May 01; 50
AB  - 1604 Objectives Overexpression of hexokinase II (HK II) that catalyzes the initial metabolic step of glycolysis is reported in various cancer cells. Here, we have evaluated the changes in F-18 FDG and H-3 PCV uptake after transfection of a vector containing micro RNA (miRNA) for HK II mRNA and HSV1-sr39 as a reporter gene in the 293 and HCT15 cells. Methods We have engineered three candidates of miRNA for isoenzymes of human Type II hexokinase (HK-II) and prepared stable cells expressing both miRNA for HK-II and GFP gene in HCT15 and ARO cells. Inhibition of HK-II expression by each miHKII was assessed by RT-PCR, western blot analysis and F-18 FDG uptake. A vector construct containing the miRNA for HK-II gene and HSV1-sr39tk cDNA coupled CMV promoters, was designed as THKII. Expression of two genes was evaluated with using F-18 FDG and H-3 PCV uptakes in 293 and HCT15 stable cells in vitro. Results In the miHKII transfected cells, the level of mRNA and protein for HK-II-1, 2 and 3 was decreased compared to parent cells. Decreased cellular uptake of F-18 FDG was observed in transfected cells and those were positively correlated with the level of HKII expression. Transfection of THKII to the 293 and HCT15 cells resulted in 14- and 4-fold increases in H-3 PCV uptake than those control cells, while decreased F-18 FDG uptake about 35% and 40% compared to control cells. Conclusions Transfection of a vector containing miRNA for HK II mRNA and HSV1-sr39 gene resulted in increased H-3 PCV uptake and decreased F-18 FDG uptake in cancer cells, and those are correlated with the level of HK-II expression.