TY - JOUR T1 - 99mTc-TRODAT-1 Micro-SPECT study on dopamine transporter in myopia retina JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1189 LP - 1189 VL - 50 IS - supplement 2 AU - Juan Zhao AU - Xiaomei Qu AU - Jia Huang AU - Yujin Qi AU - Qiusheng Dai AU - Xuezhu Zhang AU - Wei Zhou AU - Weiqing Hu AU - Xingdang Liu Y1 - 2009/05/01 UR - http://jnm.snmjournals.org/content/50/supplement_2/1189.abstract N2 - 1189 Objectives To investigate the distributions and changes of dopamine transporter (DAT) using 99mTc-TRODAT-1 in form deprivation myopia retina. Methods Pigmented guinea pigs aged 3 weeks were randomly assigned into two groups: form deprivation myopia (FDM) and normal control group. FDM group wore a translucent goggle covering randomly for 4 weeks, and both groups underwent biometric measurement before and after the experiment. At the 4th week, all the animals were performed Micro-SPECT imaging and ex vivo autoradiography with 99mTc-TRODAT-1. Results The retinas were clearly resolved in both Micro-SPECT imaging and ex vivo autoradiography. In Micro-SPECT imaging, the ratio of 99mTc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the normal control eyes (15.20±1.98, P = 0.026, F = 2.94, t = 2.605), and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04, P = 0.003, t = 5.476). In ex vivo autoradiography, the ratio of 99mTc-TRODAT-1 uptake in the myopic retina (95.52±12.04) was 18.54±5.86 lower than that in the normal control eyes (114.06±7.81, P = 0.01, F = 0.331, t = 3.164), and was 16.95±5.78 lower than that in the fellow eyes (112.47±15.67, P = 0.001, t = 7.179). Conclusions 99mTc-TRODAT-1 Micro-SPECT can be used to trace the distributions and changes of DAT in retina. Radionuclide tracing may provide a new approach for further studies on the dopamine system in the myopia. ER -