TY - JOUR T1 - [11C]SSR180575: Preliminary evaluation in rodent models of neuroinflammation JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1218 LP - 1218 VL - 50 IS - supplement 2 AU - Fabien Chauveau AU - Herve Boutin AU - Nadja Van Camp AU - Cyrille Thominiaux AU - Philippe Hantraye AU - Thomas Rooney AU - Jesus Benavides AU - Frederic Dolle AU - Bertrand Tavitian Y1 - 2009/05/01 UR - http://jnm.snmjournals.org/content/50/supplement_2/1218.abstract N2 - 1218 Objectives To evaluate the PBR ligand [11C]SSR180575 in an acute and chronic model for neuroinflammation as compared to the reference [11C]PK11195. Methods SSR180575 was labeled with carbon-11. For acute and chronic neuroinflammation we used respectively the AMPA rat model (n=14) (Boutin JNM 2007) and double transgenic mice (APP×PS1, n=15) for Alzheimer’s disease at the age of 12 months. All animals were scanned using the Concorde Focus 220 µPET scanner. Both rat and mouse models were divided in 4 equal groups to study the brain kinetics of [11C]PK11195 and [11C]SSR180575, and the specificity of the binding by displacement studies using an excess (1 mg/kg) of non-labeled PK11195 or SSR180575. Additional to in vivo studies we performed in vitro autoradiography on the same models. Results In rats, the uptake ratio of [11C]SSR180575 in the lesioned-over intact striatum was significantly higher for [11C]SSR180575 than [11C]PK11195. Competition with PK11195 or SSR180575 quickly displaced the tracer from the lesioned side. Autoradiography on brain sections confirmed the in vivo results with a high ipsi- to contralateral ratio (3.8), which was abolished by an excess of PK11195 or SSR180575. In both PS1 and APPxPS1 mice, [11C]SSR180575 uptake in the whole brain was higher as compared to the uptake of [11C]PK11195. In addition, in vitro autoradiography showed high specific binding in APPxPS1 mice that was double the binding of PS1 mice. Conclusions The results obtained confirm the potential of [11C]SSR180575 to image neuroinflammation. ER -